Gene description for Rack1
Gene name Receptor of activated protein kinase C 1
Gene symbol Rack1
Other names/aliases CG7111
DmelCG7111
RACK1
dRack1
i173
rack1
Species Drosophila melanogaster
 Database cross references - Rack1
ExoCarta ExoCarta_34070
Vesiclepedia VP_34070
Entrez Gene 34070
UniProt O18640  
 Rack1 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Rack1
Molecular Function
    structural constituent of ribosome GO:0003735 IDA
    protein-macromolecule adaptor activity GO:0030674 IPI
Biological Process
    mitophagy GO:0000423 IMP
    negative regulation of protein phosphorylation GO:0001933 IMP
    cytoplasmic translation GO:0002181 IC
    translation reinitiation GO:0002188 IGI
    locomotory behavior GO:0007626 IMP
    cellular response to starvation GO:0009267 IMP
    regulation of autophagosome size GO:0016243 IMP
    egg-laying behavior GO:0018991 IMP
    positive regulation of actin filament polymerization GO:0030838 IMP
    positive regulation of protein ubiquitination GO:0031398 IGI
    wing disc development GO:0035220 IMP
    cuticle development GO:0042335 IMP
    positive regulation by host of viral genome replication GO:0044829 IMP
    positive regulation of glycogen biosynthetic process GO:0045725 IMP
    negative regulation of smoothened signaling pathway GO:0045879 IGI
    negative regulation of smoothened signaling pathway GO:0045879 IMP
    muscle cell cellular homeostasis GO:0046716 IGI
    oogenesis GO:0048477 IMP
    IRES-dependent viral translational initiation GO:0075522 IMP
    positive regulation of protein deubiquitination GO:1903003 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    autophagosome GO:0005776 IDA
    cytosol GO:0005829 HDA
    cytosol GO:0005829 IDA
    cytosolic ribosome GO:0022626 IDA
 Experiment description of studies that identified Rack1 in exosomes
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rack1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 zuc  
Proximity Label-MS Drosophila melanogaster
2 CG14540  
Two-hybrid Drosophila melanogaster
3 CG12404  
Two-hybrid Drosophila melanogaster
4 CG6945  
Two-hybrid Drosophila melanogaster
5 Ndae1  
Two-hybrid Drosophila melanogaster
6 Fadd  
Affinity Capture-MS Drosophila melanogaster
7 Pms2  
Two-hybrid Drosophila melanogaster
8 Sgf11  
Two-hybrid Drosophila melanogaster
9 Cyp9f2  
Two-hybrid Drosophila melanogaster
10 Sry-alpha  
Two-hybrid Drosophila melanogaster
11 imd  
Two-hybrid Drosophila melanogaster
Affinity Capture-MS Drosophila melanogaster
12 msi  
Two-hybrid Drosophila melanogaster
13 CG14894  
Proximity Label-MS Drosophila melanogaster
14 Iap2  
Affinity Capture-MS Drosophila melanogaster
15 CG6660  
Two-hybrid Drosophila melanogaster
16 Sxl  
Two-hybrid Drosophila melanogaster
17 CG11380  
Two-hybrid Drosophila melanogaster
18 PGRP-LC  
Affinity Capture-MS Drosophila melanogaster
19 PAN2  
Two-hybrid Drosophila melanogaster
20 me31B  
Proximity Label-MS Drosophila melanogaster
21 atl  
Affinity Capture-MS Drosophila melanogaster
22 CG9083  
Two-hybrid Drosophila melanogaster
23 CG16985  
Two-hybrid Drosophila melanogaster
24 Rel  
Affinity Capture-MS Drosophila melanogaster
25 Prosalpha2 41531
Two-hybrid Drosophila melanogaster
26 Ubpy  
Two-hybrid Drosophila melanogaster
27 exd  
Two-hybrid Drosophila melanogaster
28 CG6950  
Two-hybrid Drosophila melanogaster
29 CTPsyn  
Proximity Label-MS Drosophila melanogaster
30 egl  
Proximity Label-MS Drosophila melanogaster
31 hpo  
Two-hybrid Drosophila melanogaster
32 CG3995  
Two-hybrid Drosophila melanogaster
33 TBPH  
Two-hybrid Drosophila melanogaster
34 CG6357  
Two-hybrid Drosophila melanogaster
35 Cpr73D  
Two-hybrid Drosophila melanogaster
36 CG3308  
Two-hybrid Drosophila melanogaster
37 CG11486  
Two-hybrid Drosophila melanogaster
38 for  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml
 Pathways in which Rack1 is involved
PathwayEvidenceSource
Death Receptor Signaling IEA Reactome
Regulation of TNFR1 signaling IEA Reactome
Signal Transduction IEA Reactome
TNF signaling IEA Reactome





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