Gene description for Rack1
Gene name Receptor of activated protein kinase C 1
Gene symbol Rack1
Other names/aliases CG7111
DmelCG7111
RACK1
dRack1
i173
rack1
Species Drosophila melanogaster
 Database cross references - Rack1
ExoCarta ExoCarta_34070
Entrez Gene 34070
UniProt O18640  
 Rack1 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Rack1
Molecular Function
    protein kinase C binding GO:0005080 NAS
Biological Process
    wing disc development GO:0035220 IMP
    oviposition GO:0018991 IMP
    locomotory behavior GO:0007626 IMP
    oogenesis GO:0048477 IMP
    IRES-dependent viral translational initiation GO:0075522 IMP
    positive regulation by host of viral genome replication GO:0044829 IMP
    muscle cell cellular homeostasis GO:0046716 IGI
    mRNA splicing, via spliceosome GO:0000398 IC
    cuticle development GO:0042335 IMP
    regulation of autophagosome size GO:0016243 IMP
    positive regulation of glycogen biosynthetic process GO:0045725 IMP
    cellular response to starvation GO:0009267 IMP
Subcellular Localization
    cytosol GO:0005829 IDA
    catalytic step 2 spliceosome GO:0071013 IDA
    autophagosome GO:0005776 IDA
    precatalytic spliceosome GO:0071011 IDA
    microtubule associated complex GO:0005875 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified Rack1 in exosomes
1
Experiment ID 202
ISEV standards
IEM
EV Biophysical techniques
TSG10|RAB35
EV Cytosolic markers
FLOT2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rack1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rack1 is involved
No pathways found





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