Gene description for Prosalpha2
Gene name Proteasome alpha2 subunit
Gene symbol Prosalpha2
Other names/aliases 153303_at
Alpha-2
CG5266
DmelCG5266
PRC3
PROS Dm25
PROS-Dm25g
PSA2_DROME
Pros25
Prot. 25 Kda
Su(DTS)
Su(DTS)-1
alpha2
alpha2_dm
dalpha2
Species Drosophila melanogaster
 Database cross references - Prosalpha2
ExoCarta ExoCarta_41531
Entrez Gene 41531
UniProt P40301  
 Prosalpha2 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Prosalpha2
Molecular Function
    endopeptidase activity GO:0004175 IC
    threonine-type endopeptidase activity GO:0004298 IEA
Biological Process
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    mitotic spindle organization GO:0007052 IMP
    mitotic spindle assembly GO:0090307 IMP
    cell proliferation GO:0008283 IMP
    mitotic spindle elongation GO:0000022 IMP
Subcellular Localization
    proteasome core complex GO:0005839 IDA
    proteasome complex GO:0000502 IDA
    proteasome core complex, alpha-subunit complex GO:0019773 IEA
 Experiment description of studies that identified Prosalpha2 in exosomes
1
Experiment ID 202
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG10|RAB35
EV Cytosolic markers
✔
FLOT2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Prosalpha2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prosalpha2 is involved
PathwayEvidenceSource
Activation of NF-kappaB in B cells IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
APC/C:Cdc20 mediated degradation of Securin IEA Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 IEA Reactome
Asymmetric localization of PCP proteins IEA Reactome
AUF1 (hnRNP D0) destabilizes mRNA IEA Reactome
Autodegradation of Cdh1 by Cdh1:APC/C IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Cross-presentation of soluble exogenous antigens (endosomes) IEA Reactome
Dectin-1 mediated noncanonical NF-kB signaling IEA Reactome
degradation of AXIN IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
degradation of DVL IEA Reactome
Degradation of GLI1 by the proteasome IEA Reactome
Degradation of GLI2 by the proteasome IEA Reactome
ER-Phagosome pathway IEA Reactome
GLI3 is processed to GLI3R by the proteasome IEA Reactome
Hedgehog 'on' state IEA Reactome
Hedgehog ligand biogenesis IEA Reactome
Orc1 removal from chromatin IEA Reactome
Orc1 removal from chromatin IEA Reactome
Regulation of ornithine decarboxylase (ODC) IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
SCF-beta-TrCP mediated degradation of Emi1 IEA Reactome
Separation of Sister Chromatids IEA Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A IEA Reactome
Ubiquitin-dependent degradation of Cyclin D1 IEA Reactome





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