Gene description for Rpn13
Gene name Regulatory particle non-ATPase 13
Gene symbol Rpn13
Other names/aliases CG13349
DmelCG13349
GC13349
p42E
Species Drosophila melanogaster
 Database cross references - Rpn13
ExoCarta ExoCarta_36545
Entrez Gene 36545
UniProt Q7K2G1  
 Rpn13 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Rpn13
Molecular Function
    ubiquitin binding GO:0043130 ISS
    endopeptidase activator activity GO:0061133 ISS
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 ISS
    lateral inhibition GO:0046331 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IC
    proteasome assembly GO:0043248 ISS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    proteasome complex GO:0000502 ISS
    nucleus GO:0005634 IDA
    proteasome regulatory particle GO:0005838 IDA
    proteasome regulatory particle, lid subcomplex GO:0008541 ISS
 Experiment description of studies that identified Rpn13 in exosomes
1
Experiment ID 202
ISEV standards
IEM
EV Biophysical techniques
TSG10|RAB35
EV Cytosolic markers
FLOT2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rpn13
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rpn13 is involved
No pathways found





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