Gene description for Rpn1
Gene name Regulatory particle non-ATPase 1
Gene symbol Rpn1
Other names/aliases BcDNA:LD21723
CG7762
DmelCG7762
RPN1
S2
p97
Species Drosophila melanogaster
 Database cross references - Rpn1
ExoCarta ExoCarta_40174
Entrez Gene 40174
 Rpn1 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Rpn1
Molecular Function
    enzyme regulator activity GO:0030234 IEA
    zinc ion binding GO:0008270 IDA
Biological Process
    regulation of protein catabolic process GO:0042176 IEA
    mitotic spindle elongation GO:0000022 IMP
    mitotic spindle organization GO:0007052 IMP
    cell proliferation GO:0008283 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
Subcellular Localization
    proteasome complex GO:0000502 IDA
    proteasome regulatory particle GO:0005838 ISS
    proteasome regulatory particle, base subcomplex GO:0008540 NAS
 Experiment description of studies that identified Rpn1 in exosomes
1
Experiment ID 202
ISEV standards
IEM
EV Biophysical techniques
TSG10|RAB35
EV Cytosolic markers
FLOT2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rpn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rpn1 is involved
No pathways found





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