Gene description for Rpn1
Gene name Regulatory particle non-ATPase 1
Gene symbol Rpn1
Other names/aliases BcDNA:LD21723
CG7762
DmelCG7762
RPN1
S2
p97
Species Drosophila melanogaster
 Database cross references - Rpn1
ExoCarta ExoCarta_40174
Vesiclepedia VP_40174
Entrez Gene 40174
 Rpn1 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Rpn1
Molecular Function
    zinc ion binding GO:0008270 IDA
Biological Process
    determination of adult lifespan GO:0008340 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IC
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
Subcellular Localization
    proteasome complex GO:0000502 IDA
    proteasome regulatory particle GO:0005838 IDA
    proteasome regulatory particle GO:0005838 ISS
    proteasome regulatory particle, base subcomplex GO:0008540 ISS
 Experiment description of studies that identified Rpn1 in exosomes
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rpn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rab7  
Affinity Capture-MS Drosophila melanogaster
2 Cyp12d1-p  
Affinity Capture-MS Drosophila melanogaster
3 Prosalpha6  
Affinity Capture-MS Drosophila melanogaster
4 Ubqn  
Reconstituted Complex Drosophila melanogaster
5 Tom20  
Proximity Label-MS Drosophila melanogaster
6 Rpn3  
Affinity Capture-MS Drosophila melanogaster
7 Prosbeta4 34999
Affinity Capture-MS Drosophila melanogaster
8 Prosbeta2 39628
Affinity Capture-MS Drosophila melanogaster
9 Rpt6  
Affinity Capture-MS Drosophila melanogaster
10 CG13319  
Affinity Capture-MS Drosophila melanogaster
11 Prosalpha1 45780
Affinity Capture-MS Drosophila melanogaster
12 Prosalpha4 32584
Affinity Capture-MS Drosophila melanogaster
13 Prosbeta5  
Affinity Capture-MS Drosophila melanogaster
14 Su(fu)  
Two-hybrid Drosophila melanogaster
15 CG11885  
Affinity Capture-MS Drosophila melanogaster
16 Rpt2 42828
Affinity Capture-MS Drosophila melanogaster
17 Gfat2  
Affinity Capture-MS Drosophila melanogaster
18 Rpt1  
Affinity Capture-MS Drosophila melanogaster
19 Chc 32537
Affinity Capture-MS Drosophila melanogaster
20 Prosalpha3 37378
Affinity Capture-MS Drosophila melanogaster
21 Pp2A-29B  
Affinity Capture-MS Drosophila melanogaster
22 Rpn2 43449
Affinity Capture-MS Drosophila melanogaster
23 Prosalpha7 36018
Affinity Capture-MS Drosophila melanogaster
24 CG7903  
Two-hybrid Drosophila melanogaster
25 wal  
Affinity Capture-MS Drosophila melanogaster
26 Prosbeta1 46058
Affinity Capture-MS Drosophila melanogaster
27 MRP  
Affinity Capture-MS Drosophila melanogaster
28 Pomp  
Affinity Capture-MS Drosophila melanogaster
29 CG2046  
Affinity Capture-MS Drosophila melanogaster
30 CG12321  
Affinity Capture-MS Drosophila melanogaster
31 CoVIb  
Two-hybrid Drosophila melanogaster
32 Rpn7  
Affinity Capture-MS Drosophila melanogaster
33 CG3523 33524
Affinity Capture-MS Drosophila melanogaster
34 Cctgamma 42029
Affinity Capture-MS Drosophila melanogaster
35 Rpn11  
Affinity Capture-MS Drosophila melanogaster
36 CG14894  
Proximity Label-MS Drosophila melanogaster
37 Ranbp9  
Affinity Capture-MS Drosophila melanogaster
38 Prosalpha2 41531
Affinity Capture-MS Drosophila melanogaster
39 Prosalpha5  
Affinity Capture-MS Drosophila melanogaster
40 Rpn5  
Affinity Capture-MS Drosophila melanogaster
41 Prosbeta6  
Affinity Capture-MS Drosophila melanogaster
42 Aats-glupro 42834
Affinity Capture-MS Drosophila melanogaster
43 Rpn13 36545
Affinity Capture-MS Drosophila melanogaster
44 CG8258 35882
Affinity Capture-MS Drosophila melanogaster
45 Rpt3  
Affinity Capture-MS Drosophila melanogaster
46 Rpn6  
Affinity Capture-MS Drosophila melanogaster
47 Rpt4  
Affinity Capture-MS Drosophila melanogaster
48 Rpn8  
Affinity Capture-MS Drosophila melanogaster
49 betaCOP  
Affinity Capture-MS Drosophila melanogaster
50 Rpn12  
Affinity Capture-MS Drosophila melanogaster
51 Rpt5  
Affinity Capture-MS Drosophila melanogaster
52 Rpn9  
Affinity Capture-MS Drosophila melanogaster
53 Prosbeta7  
Affinity Capture-MS Drosophila melanogaster
54 Cnx99A  
Affinity Capture-MS Drosophila melanogaster
55 Prosbeta3  
Affinity Capture-MS Drosophila melanogaster
View the network image/svg+xml
 Pathways in which Rpn1 is involved
No pathways found





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