Gene description for Prosalpha4
Gene name Proteasome alpha4 subunit
Gene symbol Prosalpha4
Other names/aliases 28.1Pros
3422
Alpha-4
CG3422
DmelCG3422
PROS-28.1
PROS-Dm28.1
PROSA-28.1
PSA4_DROME
Pros-Dm28.1
Pros28
Pros28.1
a4
alpha4
alpha4_dm
pros28.1
Species Drosophila melanogaster
 Database cross references - Prosalpha4
ExoCarta ExoCarta_32584
Vesiclepedia VP_32584
Entrez Gene 32584
UniProt P22769  
 Prosalpha4 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Prosalpha4
Biological Process
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IC
Subcellular Localization
    proteasome complex GO:0000502 IDA
    proteasome core complex GO:0005839 IDA
 Experiment description of studies that identified Prosalpha4 in exosomes
1
Experiment ID 202
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG10|RAB35|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Prosalpha4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CG14715  
Affinity Capture-MS Drosophila melanogaster
2 awd  
Affinity Capture-MS Drosophila melanogaster
3 CG30382 246582
Two-hybrid Drosophila melanogaster
4 CG10638  
Affinity Capture-MS Drosophila melanogaster
5 phyl  
Two-hybrid Drosophila melanogaster
6 Prosbeta4 34999
Affinity Capture-MS Drosophila melanogaster
7 Sh3beta 38820
Affinity Capture-MS Drosophila melanogaster
8 Prosbeta2 39628
Affinity Capture-MS Drosophila melanogaster
9 CG15717  
Affinity Capture-MS Drosophila melanogaster
10 Rpt4  
Affinity Capture-MS Drosophila melanogaster
11 Rpt6  
Affinity Capture-MS Drosophila melanogaster
12 CG13319  
Affinity Capture-MS Drosophila melanogaster
13 Prosalpha1 45780
Affinity Capture-MS Drosophila melanogaster
14 Prosbeta3  
Affinity Capture-MS Drosophila melanogaster
15 Prosbeta5  
Affinity Capture-MS Drosophila melanogaster
16 CG11885  
Affinity Capture-MS Drosophila melanogaster
17 Rpt2 42828
Affinity Capture-MS Drosophila melanogaster
18 Crc  
Affinity Capture-MS Drosophila melanogaster
19 Prosalpha5  
Affinity Capture-MS Drosophila melanogaster
Two-hybrid Drosophila melanogaster
20 Prosalpha6  
Affinity Capture-MS Drosophila melanogaster
21 Prosalpha3 37378
Affinity Capture-MS Drosophila melanogaster
22 Rpn2 43449
Affinity Capture-MS Drosophila melanogaster
23 Prosbeta4R2  
Affinity Capture-MS Drosophila melanogaster
24 Prosalpha7 36018
Affinity Capture-MS Drosophila melanogaster
25 CG7675  
Affinity Capture-MS Drosophila melanogaster
26 Prosbeta1 46058
Affinity Capture-MS Drosophila melanogaster
27 regucalcin  
Affinity Capture-MS Drosophila melanogaster
28 ref(2)P  
Affinity Capture-MS Drosophila melanogaster
29 Rpn9  
Affinity Capture-MS Drosophila melanogaster
30 CG12321  
Affinity Capture-MS Drosophila melanogaster
31 Rpn1 40174
Affinity Capture-MS Drosophila melanogaster
32 Rpn7  
Affinity Capture-MS Drosophila melanogaster
33 Rpn11  
Affinity Capture-MS Drosophila melanogaster
34 Klc  
Affinity Capture-MS Drosophila melanogaster
35 Prosalpha2 41531
Affinity Capture-MS Drosophila melanogaster
36 REG  
Affinity Capture-MS Drosophila melanogaster
37 CG3812  
Affinity Capture-MS Drosophila melanogaster
38 Rpt1  
Affinity Capture-MS Drosophila melanogaster
39 Fkbp14  
Affinity Capture-MS Drosophila melanogaster
40 Pdi  
Affinity Capture-MS Drosophila melanogaster
41 Prosbeta6  
Affinity Capture-MS Drosophila melanogaster
42 Rpn13 36545
Affinity Capture-MS Drosophila melanogaster
43 Rpt3  
Affinity Capture-MS Drosophila melanogaster
44 Rpn6  
Affinity Capture-MS Drosophila melanogaster
45 AnxB10  
Affinity Capture-MS Drosophila melanogaster
46 Rpn8  
Affinity Capture-MS Drosophila melanogaster
47 Rpt5  
Affinity Capture-MS Drosophila melanogaster
48 Pomp  
Affinity Capture-MS Drosophila melanogaster
49 Prosbeta7  
Affinity Capture-MS Drosophila melanogaster
50 CG6084  
Affinity Capture-MS Drosophila melanogaster
51 CG8525  
Affinity Capture-MS Drosophila melanogaster
52 CG2046  
Affinity Capture-MS Drosophila melanogaster
View the network image/svg+xml
 Pathways in which Prosalpha4 is involved
PathwayEvidenceSource
ABC-family proteins mediated transport IEA Reactome
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
Activation of NF-kappaB in B cells IEA Reactome
Adaptive Immune System IEA Reactome
Antigen processing-Cross presentation IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
APC/C-mediated degradation of cell cycle proteins IEA Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
APC/C:Cdc20 mediated degradation of Securin IEA Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 IEA Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint IEA Reactome
Asymmetric localization of PCP proteins IEA Reactome
AUF1 (hnRNP D0) binds and destabilizes mRNA IEA Reactome
Autodegradation of Cdh1 by Cdh1:APC/C IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to chemical stress IEA Reactome
Cellular response to hypoxia IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Circadian Clock pathway TAS Reactome
Class I MHC mediated antigen processing & presentation IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Cross-presentation of soluble exogenous antigens (endosomes) IEA Reactome
Cyclin A:Cdk2-associated events at S phase entry IEA Reactome
Cyclin E associated events during G1/S transition IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Dectin-1 mediated noncanonical NF-kB signaling IEA Reactome
Degradation of AXIN IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
Degradation of CRY TAS Reactome
Degradation of DVL IEA Reactome
Degradation of GLI1 by the proteasome IEA Reactome
Degradation of NF-kappa-B inhibitor, CACT TAS Reactome
Degradation of PER TAS Reactome
Degradation of TIM TAS Reactome
Deubiquitination IEA Reactome
DNA Replication IEA Reactome
Downstream signaling events of B Cell Receptor (BCR) IEA Reactome
Downstream TCR signaling IEA Reactome
Drosophila signaling pathways TAS Reactome
FBXL7 down-regulates AURKA during mitotic entry and in early mitosis IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated NF-kB activation IEA Reactome
G1/S DNA Damage Checkpoints IEA Reactome
G1/S Transition IEA Reactome
G2/M Transition IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GLI3 is processed to GLI3R by the proteasome IEA Reactome
GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 IEA Reactome
Hedgehog 'off' state IEA Reactome
Hedgehog 'on' state IEA Reactome
Hedgehog ligand biogenesis IEA Reactome
Hedgehog pathway TAS Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interleukin-1 family signaling IEA Reactome
Interleukin-1 signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
KEAP1-NFE2L2 pathway IEA Reactome
M Phase IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome
Metabolism of polyamines IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G1 phase and G1/S transition IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
N-HH ligand bound to PTC receptor complex TAS Reactome
N-HH ligand not bound to PTC receptor complex TAS Reactome
Neddylation IEA Reactome
NIK-->noncanonical NF-kB signaling IEA Reactome
Nuclear CI is degraded TAS Reactome
Nuclear events mediated by NFE2L2 IEA Reactome
Orc1 removal from chromatin IEA Reactome
Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha IEA Reactome
p53-Independent DNA Damage Response IEA Reactome
p53-Independent G1/S DNA damage checkpoint IEA Reactome
PCP/CE pathway IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Post-translational protein modification IEA Reactome
Proteasome assembly IEA Reactome
PTEN Regulation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Regulation of mitotic cell cycle IEA Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements IEA Reactome
Regulation of ornithine decarboxylase (ODC) IEA Reactome
Regulation of PTEN stability and activity IEA Reactome
Regulation of RAS by GAPs IEA Reactome
Regulation of RUNX2 expression and activity IEA Reactome
Regulation of RUNX3 expression and activity IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RUNX1 regulates transcription of genes involved in differentiation of HSCs IEA Reactome
S Phase IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by WNT IEA Reactome
Switching of origins to a post-replicative state IEA Reactome
Synthesis of DNA IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
TCR signaling IEA Reactome
TNFR2 non-canonical NF-kB pathway IEA Reactome
Toll pathway TAS Reactome
Transcriptional regulation by RUNX1 IEA Reactome
Transcriptional regulation by RUNX2 IEA Reactome
Transcriptional regulation by RUNX3 IEA Reactome
Transport of small molecules IEA Reactome
Ub-specific processing proteases IEA Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A IEA Reactome
Ubiquitin-dependent degradation of Cyclin D IEA Reactome
Ubiquitination and degradation of phosphorylated ARM TAS Reactome
Ubiquitination and proteolysis of phosphorylated CI TAS Reactome
UCH proteinases IEA Reactome
WG ligand not bound to FZ receptors TAS Reactome
Wingless pathway TAS Reactome





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