Gene description for Chc
Gene name Clathrin heavy chain
Gene symbol Chc
Other names/aliases CG9012
CHC
CLH
Cla
Clh
D-Chc
DmelCG9012
chc
chc1
dCHC
l(1)13Fb
l(1)G0438
l(1)VI
Species Drosophila melanogaster
 Database cross references - Chc
ExoCarta ExoCarta_32537
Vesiclepedia VP_32537
Entrez Gene 32537
UniProt P29742  
 Chc identified in sEVs derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Chc
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    endocytosis GO:0006897 IMP
    receptor-mediated endocytosis GO:0006898 IMP
    chemical synaptic transmission GO:0007268 IMP
    sperm individualization GO:0007291 IMP
    oocyte microtubule cytoskeleton polarization GO:0008103 IMP
    positive regulation of autophagy GO:0010508 IMP
    synaptic vesicle exocytosis GO:0016079 IMP
    extracellular matrix organization GO:0030198 IMP
    receptor internalization GO:0031623 IGI
    secretory granule organization GO:0033363 IMP
    liquid clearance, open tracheal system GO:0035002 IMP
    regulation of tube length, open tracheal system GO:0035159 IMP
    regulation of growth GO:0040008 IMP
    pole plasm oskar mRNA localization GO:0045451 IMP
    positive regulation of endocytosis GO:0045807 IMP
    compound eye retinal cell programmed cell death GO:0046667 IMP
    compound eye development GO:0048749 IMP
Subcellular Localization
    trans-Golgi network GO:0005802 IDA
    plasma membrane GO:0005886 IDA
    clathrin-coated pit GO:0005905 IDA
    cell cortex GO:0005938 IDA
    clathrin-coated vesicle GO:0030136 IDA
    secretory granule GO:0030141 IDA
    cytoplasmic vesicle GO:0031410 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified Chc in sEVs
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Chc
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nup358  
Affinity Capture-MS Drosophila melanogaster
2 Sc2  
Affinity Capture-MS Drosophila melanogaster
3 CG5854 42831
Affinity Capture-MS Drosophila melanogaster
4 Atpalpha  
Affinity Capture-MS Drosophila melanogaster
5 Tcp-1zeta 32518
Affinity Capture-MS Drosophila melanogaster
6 Rac1  
Two-hybrid Drosophila melanogaster
7 CG13220  
Affinity Capture-MS Drosophila melanogaster
8 NC2alpha  
Two-hybrid Drosophila melanogaster
9 CG8258 35882
Affinity Capture-MS Drosophila melanogaster
10 so  
Two-hybrid Drosophila melanogaster
11 Arf79F  
Affinity Capture-MS Drosophila melanogaster
12 CG2064  
Affinity Capture-MS Drosophila melanogaster
13 Tcp-1eta 41054
Affinity Capture-MS Drosophila melanogaster
14 TER94  
Affinity Capture-MS Drosophila melanogaster
15 Pp2A-29B  
Affinity Capture-MS Drosophila melanogaster
16 Psa  
Affinity Capture-MS Drosophila melanogaster
17 Gp210  
Affinity Capture-MS Drosophila melanogaster
18 Gp93  
Affinity Capture-MS Drosophila melanogaster
19 Cdk5  
Two-hybrid Drosophila melanogaster
20 CG3523 33524
Affinity Capture-MS Drosophila melanogaster
21 Ranbp9  
Affinity Capture-MS Drosophila melanogaster
22 slmb  
Two-hybrid Drosophila melanogaster
23 Rpt5  
Affinity Capture-MS Drosophila melanogaster
24 CG12264  
Affinity Capture-MS Drosophila melanogaster
25 CG5525  
Affinity Capture-MS Drosophila melanogaster
26 CG7033  
Affinity Capture-MS Drosophila melanogaster
27 Cyp12d1-p  
Affinity Capture-MS Drosophila melanogaster
28 Rpt6  
Affinity Capture-MS Drosophila melanogaster
29 ImpL3 45880
Affinity Capture-MS Drosophila melanogaster
30 CG33303  
Affinity Capture-MS Drosophila melanogaster
31 Rpt1  
Affinity Capture-MS Drosophila melanogaster
32 Ost48  
Affinity Capture-MS Drosophila melanogaster
33 CG1444  
Affinity Capture-MS Drosophila melanogaster
34 Pi3K68D  
Affinity Capture-MS Drosophila melanogaster
35 MRP  
Affinity Capture-MS Drosophila melanogaster
36 Bap60  
Two-hybrid Drosophila melanogaster
37 CG31999  
Two-hybrid Drosophila melanogaster
38 Rpt2 42828
Affinity Capture-MS Drosophila melanogaster
39 CG12262  
Affinity Capture-MS Drosophila melanogaster
40 Kap-alpha3  
Affinity Capture-MS Drosophila melanogaster
41 CG1416 35426
Affinity Capture-MS Drosophila melanogaster
42 Rab1  
Affinity Capture-MS Drosophila melanogaster
43 Gfat2  
Affinity Capture-MS Drosophila melanogaster
44 OstDelta  
Affinity Capture-MS Drosophila melanogaster
45 bor  
Affinity Capture-MS Drosophila melanogaster
46 Rab5 33418
Affinity Capture-MS Drosophila melanogaster
47 boca  
Affinity Capture-MS Drosophila melanogaster
48 Karybeta3  
Affinity Capture-MS Drosophila melanogaster
49 CG17259  
Affinity Capture-MS Drosophila melanogaster
50 pUf68  
Two-hybrid Drosophila melanogaster
51 CG11882  
Two-hybrid Drosophila melanogaster
Affinity Capture-MS Drosophila melanogaster
52 Sucb  
Affinity Capture-MS Drosophila melanogaster
53 Vha68-2  
Affinity Capture-MS Drosophila melanogaster
54 Hrs  
Two-hybrid Drosophila melanogaster
55 Rpn1 40174
Affinity Capture-MS Drosophila melanogaster
56 Kr-h2  
Affinity Capture-MS Drosophila melanogaster
57 Cctgamma 42029
Affinity Capture-MS Drosophila melanogaster
58 ATPsyn-gamma  
Affinity Capture-MS Drosophila melanogaster
59 Nnf1a  
Two-hybrid Drosophila melanogaster
60 CG11327  
Two-hybrid Drosophila melanogaster
61 Rop  
Affinity Capture-MS Drosophila melanogaster
62 betaCOP  
Affinity Capture-MS Drosophila melanogaster
63 EfTuM  
Affinity Capture-MS Drosophila melanogaster
64 l(1)G0156  
Affinity Capture-MS Drosophila melanogaster
65 CG3008  
Affinity Capture-MS Drosophila melanogaster
66 Es2  
Two-hybrid Drosophila melanogaster
67 CG4169  
Affinity Capture-MS Drosophila melanogaster
68 r  
Affinity Capture-MS Drosophila melanogaster
69 Clc  
Affinity Capture-MS Drosophila melanogaster
70 Ca-P60A  
Affinity Capture-MS Drosophila melanogaster
71 Past1  
Affinity Capture-MS Drosophila melanogaster
72 Mpcp  
Affinity Capture-MS Drosophila melanogaster
73 blw  
Affinity Capture-MS Drosophila melanogaster
74 ci  
Two-hybrid Drosophila melanogaster
75 CG2789  
Affinity Capture-MS Drosophila melanogaster
76 wal  
Affinity Capture-MS Drosophila melanogaster
77 alpha-Spec  
Affinity Capture-MS Drosophila melanogaster
78 Sxl  
Two-hybrid Drosophila melanogaster
79 Glt  
Affinity Capture-MS Drosophila melanogaster
80 CG1516  
Affinity Capture-MS Drosophila melanogaster
81 Mec2 32905
Affinity Capture-MS Drosophila melanogaster
82 Aldh  
Affinity Capture-MS Drosophila melanogaster
83 egl  
Proximity Label-MS Drosophila melanogaster
84 Aats-glupro 42834
Affinity Capture-MS Drosophila melanogaster
85 Hsp83  
Affinity Capture-MS Drosophila melanogaster
86 Gale 38076
Affinity Capture-MS Drosophila melanogaster
87 Gie  
Affinity Capture-MS Drosophila melanogaster
88 RpLP0  
Affinity Capture-MS Drosophila melanogaster
View the network image/svg+xml
 Pathways in which Chc is involved
PathwayEvidenceSource
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Developmental Biology IEA Reactome
EPH-ephrin mediated repulsion of cells IEA Reactome
EPH-Ephrin signaling IEA Reactome
Formation of annular gap junctions IEA Reactome
Gap junction degradation IEA Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking and regulation IEA Reactome
Golgi Associated Vesicle Biogenesis IEA Reactome
L1CAM interactions IEA Reactome
LDL clearance IEA Reactome
Lysosome Vesicle Biogenesis IEA Reactome
Membrane Trafficking IEA Reactome
Nervous system development IEA Reactome
PCP/CE pathway IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance IEA Reactome
Recycling pathway of L1 IEA Reactome
Retrograde neurotrophin signalling IEA Reactome
RHO GTPase cycle IEA Reactome
RHOU GTPase cycle IEA Reactome
RHOV GTPase cycle IEA Reactome
Signal Transduction IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by WNT IEA Reactome
trans-Golgi Network Vesicle Budding IEA Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport IEA Reactome
VLDLR internalisation and degradation IEA Reactome
WNT5A-dependent internalization of FZD2, FZD5 and ROR2 IEA Reactome
WNT5A-dependent internalization of FZD4 IEA Reactome





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