Gene description for Chc
Gene name Clathrin heavy chain
Gene symbol Chc
Other names/aliases CG9012
CHC
CLH
Cla
Clh
D-Chc
DmelCG9012
chc
chc1
dCHC
l(1)13Fb
l(1)G0438
l(1)VI
Species Drosophila melanogaster
 Database cross references - Chc
ExoCarta ExoCarta_32537
Entrez Gene 32537
UniProt P29742  
 Chc identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Chc
Molecular Function
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
    clathrin light chain binding GO:0032051 ISS
Biological Process
    compound eye retinal cell programmed cell death GO:0046667 IMP
    regulation of growth GO:0040008 IMP
    synaptic vesicle exocytosis GO:0016079 IMP
    dsRNA transport GO:0033227 IMP
    synaptic vesicle coating GO:0016183 TAS
    compound eye development GO:0048749 IMP
    positive regulation of Notch signaling pathway GO:0045747 IMP
    liquid clearance, open tracheal system GO:0035002 IMP
    neurotransmitter secretion GO:0007269 NAS
    intracellular protein transport GO:0006886 IEA
    oocyte microtubule cytoskeleton polarization GO:0008103 IMP
    synaptic transmission GO:0007268 IMP
    puparial adhesion GO:0007594 IMP
    regulation of tube length, open tracheal system GO:0035159 IMP
    extracellular matrix organization GO:0030198 IMP
    positive regulation of endocytosis GO:0045807 IMP
    secretory granule organization GO:0033363 IMP
    endocytosis GO:0006897 IMP
    sperm individualization GO:0007291 IMP
    pole plasm oskar mRNA localization GO:0045451 IMP
Subcellular Localization
    coated pit GO:0005905 TAS
    cell cortex GO:0005938 IDA
    trans-Golgi network GO:0005802 IDA
    synaptic vesicle GO:0008021 TAS
    clathrin coat of coated pit GO:0030132 NAS
    clathrin-coated vesicle GO:0030136 IDA
    coated vesicle GO:0030135 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
    plasma membrane GO:0005886 IDA
    clathrin coat of trans-Golgi network vesicle GO:0030130 IEA
    clathrin vesicle coat GO:0030125 TAS
    secretory granule GO:0030141 IDA
    cytoplasmic vesicle GO:0031410 IDA
    lipid particle GO:0005811 IDA
    clathrin coat of synaptic vesicle GO:0030129 NAS
    clathrin complex GO:0071439 ISS
 Experiment description of studies that identified Chc in exosomes
1
Experiment ID 202
ISEV standards
IEM
EV Biophysical techniques
TSG10|RAB35
EV Cytosolic markers
FLOT2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Chc
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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