Gene description for Rab5
Gene name CG3664 gene product from transcript CG3664-RC
Gene symbol Rab5
Other names/aliases AAF51265
BAA88244
CG3664
DRab5
Dm Rab5
DmRab5
DmelCG3664
Drab5
RAB5
RAB5a
Rab
Rab-5
dRab5
l(2)k08232
rab5
Species Drosophila melanogaster
 Database cross references - Rab5
ExoCarta ExoCarta_33418
Vesiclepedia VP_33418
Entrez Gene 33418
 Rab5 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Rab5
Molecular Function
    protein binding GO:0005515 IPI
    enzyme regulator activity GO:0030234 IDA
Biological Process
    endocytosis GO:0006897 IMP
    receptor-mediated endocytosis GO:0006898 IMP
    endosome organization GO:0007032 IMP
    Notch receptor processing GO:0007220 IMP
    border follicle cell migration GO:0007298 IMP
    cellularization GO:0007349 IMP
    nervous system development GO:0007399 IMP
    axonogenesis GO:0007409 IMP
    synaptic vesicle to endosome fusion GO:0016189 IMP
    synaptic vesicle to endosome fusion GO:0016189 TAS
    endosome transport via multivesicular body sorting pathway GO:0032509 IMP
    endosomal vesicle fusion GO:0034058 IMP
    establishment or maintenance of apical/basal cell polarity GO:0035088 IMP
    wing disc development GO:0035220 IMP
    multivesicular body assembly GO:0036258 IMP
    dorsal closure, amnioserosa morphology change GO:0046664 IMP
    symbiont entry into host cell GO:0046718 HMP
    plasma membrane to endosome transport GO:0048227 IMP
    oogenesis GO:0048477 IMP
    synaptic vesicle endocytosis GO:0048488 IGI
    synaptic vesicle endocytosis GO:0048488 TAS
    axon extension GO:0048675 IMP
    imaginal disc-derived male genitalia morphogenesis GO:0048803 IMP
    dendrite morphogenesis GO:0048813 IMP
    regulation of synapse structure or activity GO:0050803 IMP
    clathrin-dependent endocytosis involved in vitellogenesis GO:0061883 IDA
Subcellular Localization
    early endosome GO:0005769 IDA
    early endosome GO:0005769 IDA
    early endosome GO:0005769 NAS
    plasma membrane GO:0005886 HDA
    cell cortex GO:0005938 IDA
    synaptic vesicle GO:0008021 IDA
    multivesicular body membrane GO:0032585 IDA
    neuronal cell body GO:0043025 HDA
    terminal bouton GO:0043195 IDA
    axon terminus GO:0043679 IDA
    synapse GO:0045202 HDA
    endocytic patch GO:0061645 IDA
    presynaptic endosome GO:0098830 IDA
 Experiment description of studies that identified Rab5 in exosomes
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rab5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Droj2  
Affinity Capture-MS Drosophila melanogaster
2 Sc2  
Affinity Capture-MS Drosophila melanogaster
3 CG1444  
Affinity Capture-MS Drosophila melanogaster
4 ATPsyn-gamma  
Affinity Capture-MS Drosophila melanogaster
5 ImpL3 45880
Affinity Capture-MS Drosophila melanogaster
6 Tcp-1zeta 32518
Affinity Capture-MS Drosophila melanogaster
7 COX2  
Affinity Capture-MS Drosophila melanogaster
8 Sirt4  
Two-hybrid Drosophila melanogaster
9 CG4169  
Affinity Capture-MS Drosophila melanogaster
10 R  
Affinity Capture-MS Drosophila melanogaster
11 Tapdelta  
Affinity Capture-MS Drosophila melanogaster
12 Arf79F  
Affinity Capture-MS Drosophila melanogaster
13 CG2064  
Affinity Capture-MS Drosophila melanogaster
14 Rpt1  
Affinity Capture-MS Drosophila melanogaster
15 Gfat2  
Affinity Capture-MS Drosophila melanogaster
16 Spt-I  
Affinity Capture-MS Drosophila melanogaster
17 Rab2  
Affinity Capture-MS Drosophila melanogaster
18 Chc 32537
Affinity Capture-MS Drosophila melanogaster
19 Galphao  
Two-hybrid Drosophila melanogaster
20 Sec61alpha  
Affinity Capture-MS Drosophila melanogaster
21 CG10425  
Affinity Capture-MS Drosophila melanogaster
22 cathD 45268
Affinity Capture-MS Drosophila melanogaster
23 Cyp12d1-p  
Affinity Capture-MS Drosophila melanogaster
24 CG33303  
Affinity Capture-MS Drosophila melanogaster
25 CG14894  
Proximity Label-MS Drosophila melanogaster
26 MRP  
Affinity Capture-MS Drosophila melanogaster
27 Vha68-2  
Affinity Capture-MS Drosophila melanogaster
28 SsRbeta  
Affinity Capture-MS Drosophila melanogaster
29 Hel25E  
Affinity Capture-MS Drosophila melanogaster
30 Gp93  
Affinity Capture-MS Drosophila melanogaster
31 CG12262  
Affinity Capture-MS Drosophila melanogaster
32 Mec2 32905
Affinity Capture-MS Drosophila melanogaster
33 Lrrk  
Affinity Capture-Western Drosophila melanogaster
34 me31B  
Proximity Label-MS Drosophila melanogaster
35 CG2789  
Affinity Capture-MS Drosophila melanogaster
36 Rab7  
Affinity Capture-MS Drosophila melanogaster
37 Rab6  
Affinity Capture-MS Drosophila melanogaster
38 skap  
Affinity Capture-MS Drosophila melanogaster
39 LRRK2 120892
Affinity Capture-MS Homo sapiens
40 Ca-P60A  
Affinity Capture-MS Drosophila melanogaster
41 wal  
Affinity Capture-MS Drosophila melanogaster
42 Ranbp9  
Affinity Capture-MS Drosophila melanogaster
43 CG1416 35426
Affinity Capture-MS Drosophila melanogaster
44 OstDelta  
Affinity Capture-MS Drosophila melanogaster
45 Cctgamma 42029
Affinity Capture-MS Drosophila melanogaster
46 fax  
Affinity Capture-MS Drosophila melanogaster
47 Ost48  
Affinity Capture-MS Drosophila melanogaster
48 KdelR  
Affinity Capture-MS Drosophila melanogaster
49 ox  
Affinity Capture-MS Drosophila melanogaster
50 l(1)G0156  
Affinity Capture-MS Drosophila melanogaster
51 CG12264  
Affinity Capture-MS Drosophila melanogaster
52 ZnT63C  
Affinity Capture-MS Drosophila melanogaster
53 Cnx99A  
Affinity Capture-MS Drosophila melanogaster
54 Gie  
Affinity Capture-MS Drosophila melanogaster
55 Rab1  
Affinity Capture-MS Drosophila melanogaster
56 CG4729  
Affinity Capture-MS Drosophila melanogaster
57 Cp1  
Affinity Capture-MS Drosophila melanogaster
58 Sucb  
Affinity Capture-MS Drosophila melanogaster
59 CG15098  
Affinity Capture-MS Drosophila melanogaster
60 Gdi 34264
Affinity Capture-MS Drosophila melanogaster
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