Gene description for ImpL3
Gene name Ecdysone-inducible gene L3
Gene symbol ImpL3
Other names/aliases CG10160
DmelCG10160
IMP-L3
IMPL3
Imp-L3
Impl3
LDH
Ld
Ldh
dLDH
dLdha
dmLDH
Species Drosophila melanogaster
 Database cross references - ImpL3
ExoCarta ExoCarta_45880
Vesiclepedia VP_45880
Entrez Gene 45880
UniProt Q95028  
 ImpL3 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for ImpL3
Molecular Function
    L-lactate dehydrogenase activity GO:0004459 IDA
    alpha-ketoglutarate reductase activity GO:0061759 IDA
Biological Process
    glycolytic process GO:0006096 IGI
    NADH oxidation GO:0006116 IMP
    NADH metabolic process GO:0006734 IMP
    lactate biosynthetic process from pyruvate GO:0019244 IDA
    lactate biosynthetic process from pyruvate GO:0019244 IMP
    pyruvate biosynthetic process GO:0042866 IDA
    pyruvate catabolic process GO:0042867 IDA
    lactate catabolic process GO:1903457 IDA
Subcellular Localization
    cytosol GO:0005829 IDA
 Experiment description of studies that identified ImpL3 in exosomes
1
Experiment ID 202
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG10|RAB35|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ImpL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Droj2  
Affinity Capture-MS Drosophila melanogaster
2 Sc2  
Affinity Capture-MS Drosophila melanogaster
3 CG5854 42831
Affinity Capture-MS Drosophila melanogaster
4 CG13510  
Two-hybrid Drosophila melanogaster
5 Rab5 33418
Affinity Capture-MS Drosophila melanogaster
6 Tcp-1zeta 32518
Affinity Capture-MS Drosophila melanogaster
7 R  
Affinity Capture-MS Drosophila melanogaster
8 Kr-h2  
Affinity Capture-MS Drosophila melanogaster
9 CG4169  
Affinity Capture-MS Drosophila melanogaster
10 Rpt6  
Affinity Capture-MS Drosophila melanogaster
11 CG7357  
Two-hybrid Drosophila melanogaster
12 CG33303  
Affinity Capture-MS Drosophila melanogaster
13 Arf79F  
Affinity Capture-MS Drosophila melanogaster
14 Rap2l  
Affinity Capture-MS Drosophila melanogaster
15 CG2064  
Affinity Capture-MS Drosophila melanogaster
16 Rpt1  
Affinity Capture-MS Drosophila melanogaster
17 Tapdelta  
Affinity Capture-MS Drosophila melanogaster
18 ox  
Affinity Capture-MS Drosophila melanogaster
19 Ost48  
Affinity Capture-MS Drosophila melanogaster
20 Chc 32537
Affinity Capture-MS Drosophila melanogaster
21 CG1444  
Affinity Capture-MS Drosophila melanogaster
22 CG10425  
Affinity Capture-MS Drosophila melanogaster
23 blw  
Affinity Capture-MS Drosophila melanogaster
24 CG2789  
Affinity Capture-MS Drosophila melanogaster
25 Ca-P60A  
Affinity Capture-MS Drosophila melanogaster
26 wal  
Affinity Capture-MS Drosophila melanogaster
27 Art8  
Affinity Capture-MS Drosophila melanogaster
28 CG2065  
Affinity Capture-MS Drosophila melanogaster
29 CG6453  
Affinity Capture-MS Drosophila melanogaster
30 fra  
Affinity Capture-MS Drosophila melanogaster
31 SsRbeta  
Affinity Capture-MS Drosophila melanogaster
32 Hel25E  
Affinity Capture-MS Drosophila melanogaster
33 Gp93  
Affinity Capture-MS Drosophila melanogaster
34 CG12262  
Affinity Capture-MS Drosophila melanogaster
35 Mec2 32905
Affinity Capture-MS Drosophila melanogaster
36 CG1416 35426
Affinity Capture-MS Drosophila melanogaster
37 Cctgamma 42029
Affinity Capture-MS Drosophila melanogaster
38 skap  
Affinity Capture-MS Drosophila melanogaster
39 ATPsyn-gamma  
Affinity Capture-MS Drosophila melanogaster
40 Sec13 44437
Affinity Capture-MS Drosophila melanogaster
41 PyK  
Affinity Capture-MS Drosophila melanogaster
42 OstDelta  
Affinity Capture-MS Drosophila melanogaster
43 CG17029  
Two-hybrid Drosophila melanogaster
Two-hybrid Drosophila melanogaster
44 CG11327  
Two-hybrid Drosophila melanogaster
45 CG5885  
Affinity Capture-MS Drosophila melanogaster
46 cathD 45268
Affinity Capture-MS Drosophila melanogaster
47 porin  
Affinity Capture-MS Drosophila melanogaster
48 KdelR  
Affinity Capture-MS Drosophila melanogaster
49 Rab2  
Affinity Capture-MS Drosophila melanogaster
50 Gale 38076
Affinity Capture-MS Drosophila melanogaster
51 Arf102F  
Affinity Capture-MS Drosophila melanogaster
52 Gie  
Affinity Capture-MS Drosophila melanogaster
53 Rab1  
Affinity Capture-MS Drosophila melanogaster
54 Sucb  
Affinity Capture-MS Drosophila melanogaster
55 CG15098  
Affinity Capture-MS Drosophila melanogaster
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