Gene description for Gale
Gene name UDP-galactose 4'-epimerase
Gene symbol Gale
Other names/aliases CG12030
DmelCG12030
GALE
dGALE
Species Drosophila melanogaster
 Database cross references - Gale
ExoCarta ExoCarta_38076
Vesiclepedia VP_38076
Entrez Gene 38076
UniProt Q9W0P5  
 Gale identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Gale
Molecular Function
    UDP-N-acetylglucosamine 4-epimerase activity GO:0003974 IMP
    UDP-glucose 4-epimerase activity GO:0003978 IMP
Biological Process
    UDP-alpha-D-glucose metabolic process GO:0006011 IMP
    UDP-N-acetylglucosamine metabolic process GO:0006047 IMP
    UDP-N-acetylgalactosamine metabolic process GO:0019276 IMP
    galactose catabolic process via UDP-galactose GO:0033499 IMP
    larval lymph gland hemopoiesis GO:0035167 IMP
    UDP-D-galactose metabolic process GO:0052573 IMP
 Experiment description of studies that identified Gale in exosomes
1
Experiment ID 202
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG10|RAB35|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Gale
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ImpL3 45880
Affinity Capture-MS Drosophila melanogaster
2 Tcp-1zeta 32518
Affinity Capture-MS Drosophila melanogaster
3 CG13220  
Affinity Capture-MS Drosophila melanogaster
4 CG4169  
Affinity Capture-MS Drosophila melanogaster
5 Dp1  
Affinity Capture-MS Drosophila melanogaster
6 Arf79F  
Affinity Capture-MS Drosophila melanogaster
7 CG2064  
Affinity Capture-MS Drosophila melanogaster
8 Rpt1  
Affinity Capture-MS Drosophila melanogaster
9 Rab2  
Affinity Capture-MS Drosophila melanogaster
10 Chc 32537
Affinity Capture-MS Drosophila melanogaster
11 CG1444  
Affinity Capture-MS Drosophila melanogaster
12 wal  
Affinity Capture-MS Drosophila melanogaster
13 CG12262  
Affinity Capture-MS Drosophila melanogaster
14 Kap-alpha3  
Affinity Capture-MS Drosophila melanogaster
15 CG1416 35426
Affinity Capture-MS Drosophila melanogaster
16 Aldh  
Affinity Capture-MS Drosophila melanogaster
17 Mec2 32905
Affinity Capture-MS Drosophila melanogaster
18 PGRP-LC  
Affinity Capture-MS Drosophila melanogaster
19 Gale 38076
Two-hybrid Drosophila melanogaster
Two-hybrid Drosophila melanogaster
20 CG12264  
Affinity Capture-MS Drosophila melanogaster
21 CG6685  
Two-hybrid Drosophila melanogaster
22 Gie  
Affinity Capture-MS Drosophila melanogaster
23 Rab1  
Affinity Capture-MS Drosophila melanogaster
24 Sucb  
Affinity Capture-MS Drosophila melanogaster
View the network image/svg+xml
 Pathways in which Gale is involved
PathwayEvidenceSource
Galactose catabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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