Gene description for Sec13
Gene name Sec13 ortholog (S. cerevisiae)
Gene symbol Sec13
Other names/aliases BEST:CK00043
BcDNA:LD03471
CG6773
CK00043
DmelCG6773
Dsec13
SEC13
l(3)01031
sec13
Species Drosophila melanogaster
 Database cross references - Sec13
ExoCarta ExoCarta_44437
Vesiclepedia VP_44437
Entrez Gene 44437
 Sec13 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Sec13
Molecular Function
    chromatin binding GO:0003682 IDA
    chromatin binding GO:0003682 IMP
Biological Process
    protein import into nucleus GO:0006606 IMP
    NLS-bearing protein import into nucleus GO:0006607 IMP
    larval chitin-based cuticle development GO:0008363 IMP
    cellular response to heat GO:0034605 IMP
    ecdysone-mediated polytene chromosome puffing GO:0035077 IDA
    chitin-based larval cuticle pattern formation GO:0035293 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 IMP
Subcellular Localization
    chromatin GO:0000785 IDA
    chromatin GO:0000785 IMP
    nucleoplasm GO:0005654 IDA
    polytene chromosome puff GO:0005703 IDA
    Seh1-associated complex GO:0035859 IDA
    GATOR2 complex GO:0061700 TAS
 Experiment description of studies that identified Sec13 in exosomes
1
Experiment ID 202
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG10|RAB35|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Sec13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sec31  
Two-hybrid Drosophila melanogaster
2 ImpL3 45880
Affinity Capture-MS Drosophila melanogaster
3 CG11327  
Two-hybrid Drosophila melanogaster
4 Sucb  
Affinity Capture-MS Drosophila melanogaster
5 CG12262  
Affinity Capture-MS Drosophila melanogaster
6 CG17162  
Two-hybrid Drosophila melanogaster
7 Hmg-2  
Two-hybrid Drosophila melanogaster
8 CG2789  
Affinity Capture-MS Drosophila melanogaster
9 CG3795  
Two-hybrid Drosophila melanogaster
10 otp  
Two-hybrid Drosophila melanogaster
11 CG11165  
Two-hybrid Drosophila melanogaster
12 Ipk2  
Two-hybrid Drosophila melanogaster
13 wal  
Affinity Capture-MS Drosophila melanogaster
14 CG1416 35426
Affinity Capture-MS Drosophila melanogaster
View the network image/svg+xml
 Pathways in which Sec13 is involved
PathwayEvidenceSource
Asparagine N-linked glycosylation IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
COPII-mediated vesicle transport IEA Reactome
ER to Golgi Anterograde Transport IEA Reactome
Gene expression (Transcription) IEA Reactome
Gene Silencing by RNA IEA Reactome
M Phase IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prophase IEA Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Nuclear Envelope Breakdown IEA Reactome
Nuclear Pore Complex (NPC) Disassembly IEA Reactome
Post-translational protein modification IEA Reactome
Postmitotic nuclear pore complex (NPC) reformation IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA IEA Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation IEA Reactome
SUMOylation of chromatin organization proteins IEA Reactome
SUMOylation of DNA damage response and repair proteins IEA Reactome
SUMOylation of SUMOylation proteins IEA Reactome
Transcriptional regulation by small RNAs IEA Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript IEA Reactome
Transport of Mature mRNA Derived from an Intronless Transcript IEA Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts IEA Reactome
Transport of Mature Transcript to Cytoplasm IEA Reactome
Transport of the SLBP Dependant Mature mRNA IEA Reactome
Transport of the SLBP independent Mature mRNA IEA Reactome
Transport to the Golgi and subsequent modification IEA Reactome
Vesicle-mediated transport IEA Reactome





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