Gene description for Aats-asn
Gene name Asparaginyl-tRNA synthetase
Gene symbol Aats-asn
Other names/aliases AsnRS
BcDNA:GH06451
CG10687
Ca
DmelCG10687
NRS
l(2)37Ca
l(2)37Ca)E11
l(2)E11
Species Drosophila melanogaster
 Database cross references - Aats-asn
ExoCarta ExoCarta_35194
Vesiclepedia VP_35194
Entrez Gene 35194
 Aats-asn identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Aats-asn
Molecular Function
    asparagine-tRNA ligase activity GO:0004816 TAS
Biological Process
    asparaginyl-tRNA aminoacylation GO:0006421 TAS
Subcellular Localization
    cytoplasm GO:0005737 TAS
 Experiment description of studies that identified Aats-asn in exosomes
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Aats-asn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CTPsyn  
Proximity Label-MS Drosophila melanogaster
2 Rpt1  
Affinity Capture-MS Drosophila melanogaster
3 Tcp-1zeta 32518
Affinity Capture-MS Drosophila melanogaster
4 sav  
Two-hybrid Drosophila melanogaster
5 me31B  
Proximity Label-MS Drosophila melanogaster
6 CG4169  
Affinity Capture-MS Drosophila melanogaster
7 CG14894  
Proximity Label-MS Drosophila melanogaster
8 CG1416 35426
Affinity Capture-MS Drosophila melanogaster
9 Sucb  
Affinity Capture-MS Drosophila melanogaster
View the network image/svg+xml
 Pathways in which Aats-asn is involved
No pathways found





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