Gene description for AGO2
Gene name Argonaute 2
Gene symbol AGO2
Other names/aliases AG02
AGO 2
Ago-2
Ago2
CG13452
CG7439
Dm Ago2
DmAgo2
DmelCG7439
ago
ago-2
ago2
dAGO2
dAgo2
eIF2C2
Species Drosophila melanogaster
 Database cross references - AGO2
ExoCarta ExoCarta_39683
Entrez Gene 39683
UniProt Q9VUQ5  
 AGO2 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for AGO2
Molecular Function
    protein binding GO:0005515 IPI
    endoribonuclease activity GO:0004521 IDA
    siRNA binding GO:0035197 IDA
    translation initiation factor activity GO:0003743 ISS
Biological Process
    targeting of mRNA for destruction involved in RNA interference GO:0030423 TAS
    pole cell formation GO:0007279 IMP
    production of siRNA involved in RNA interference GO:0030422 IMP
    salivary gland cell autophagic cell death GO:0035071 IEP
    syncytial nuclear migration GO:0035190 IMP
    translational initiation GO:0006413 ISS
    cellularization GO:0007349 IMP
    defense response to virus GO:0051607 IMP
    negative regulation of viral genome replication GO:0045071 IMP
    autophagic cell death GO:0048102 IEP
    dosage compensation by hyperactivation of X chromosome GO:0009047 IGI
    heterochromatin organization involved in chromatin silencing GO:0070868 IMP
    RNA interference GO:0016246 TAS
    siRNA loading onto RISC involved in RNA interference GO:0035087 IMP
    segment polarity determination GO:0007367 IGI
    dsRNA transport GO:0033227 IMP
    gene silencing by miRNA GO:0035195 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    RISC complex GO:0016442 TAS
    RISC-loading complex GO:0070578 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified AGO2 in exosomes
1
Experiment ID 202
ISEV standards
IEM
EV Biophysical techniques
TSG10|RAB35
EV Cytosolic markers
FLOT2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AGO2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which AGO2 is involved
No pathways found





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