Experiment description of studies that identified TSG101 in exosomes

1 Experiment ID 202 MISEV standards ✔ IEM Biophysical techniques ✔ TSG10|RAB35|FLOT2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23035643     Organism Drosophila melanogaster Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes. Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP." Journal name Traffic Publication year 2012 Sample Drosophila S2 cells Sample name Normal-Drosophila S2 tub-Wg Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 1227 MISEV standards ✘ Biophysical techniques ✔ TSG101|Rab11 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 31666321     Organism Drosophila melanogaster Experiment description Glia-derived exosomal miR-274 targets Sprouty in trachea and synaptic boutons to modulate growth and response to hypoxia Authors "Tsai YW, Sung HH, Li JC, Yeh CY, Chen PY, Cheng YJ, Chen CH, Tsai YC, Chien CT. " Journal name Proc Natl Acad Sci U S A Publication year 2019 Sample Embryonic cells Sample name S2 Isolation/purification methods Differential centrifugation ExoQuick Kit Flotation density - Molecules identified in the study Protein miRNA Methods used in the study RT-PCR Western blotting

3 Experiment ID 1228 MISEV standards ✘ Biophysical techniques ✔ TSG101|Rab11 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 31666321     Organism Drosophila melanogaster Experiment description Glia-derived exosomal miR-274 targets Sprouty in trachea and synaptic boutons to modulate growth and response to hypoxia Authors "Tsai YW, Sung HH, Li JC, Yeh CY, Chen PY, Cheng YJ, Chen CH, Tsai YC, Chien CT. " Journal name Proc Natl Acad Sci U S A Publication year 2019 Sample Hemolymph Sample name Circulating hemolymph Isolation/purification methods Differential centrifugation ExoQuick Kit Flotation density - Molecules identified in the study Protein miRNA Methods used in the study RT-PCR Western blotting

4 Experiment ID 1229 MISEV standards ✘ Biophysical techniques ✔ TSG101|Rab11 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 31666321     Organism Drosophila melanogaster Experiment description Glia-derived exosomal miR-274 targets Sprouty in trachea and synaptic boutons to modulate growth and response to hypoxia Authors "Tsai YW, Sung HH, Li JC, Yeh CY, Chen PY, Cheng YJ, Chen CH, Tsai YC, Chien CT. " Journal name Proc Natl Acad Sci U S A Publication year 2019 Sample Hemolymph Sample name Circulating hemolymph Isolation/purification methods Differential centrifugation ExoQuick Kit Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

Protein-protein interactions for TSG101

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CG32809   Two-hybrid Drosophila melanogaster 2 CG15676   Two-hybrid Drosophila melanogaster 3 Msp300   Two-hybrid Drosophila melanogaster 4 CG8398   Two-hybrid Drosophila melanogaster 5 CG11248   Two-hybrid Drosophila melanogaster 6 mod(r)   Two-hybrid Drosophila melanogaster 7 mud   Two-hybrid Drosophila melanogaster 8 cdi   Two-hybrid Drosophila melanogaster 9 Actn   Two-hybrid Drosophila melanogaster 10 rhea   Two-hybrid Drosophila melanogaster 11 Vps28   Affinity Capture-MS Drosophila melanogaster Affinity Capture-Western Drosophila melanogaster Two-hybrid Drosophila melanogaster 12 prominin-like   Two-hybrid Drosophila melanogaster 13 CG4364   Two-hybrid Drosophila melanogaster 14 mus210   Two-hybrid Drosophila melanogaster 15 Mnn1   Two-hybrid Drosophila melanogaster 16 Ada2b   Two-hybrid Drosophila melanogaster 17 nsl1   Two-hybrid Drosophila melanogaster 18 insc   Two-hybrid Drosophila melanogaster 19 kst   Two-hybrid Drosophila melanogaster 20 CG18858   Two-hybrid Drosophila melanogaster 21 Kat60   Two-hybrid Drosophila melanogaster 22 CG5830   Two-hybrid Drosophila melanogaster 23 CG2641   Affinity Capture-MS Drosophila melanogaster Two-hybrid Drosophila melanogaster 24 CG7192   Affinity Capture-MS Drosophila melanogaster 25 beta-Spec   Two-hybrid Drosophila melanogaster 26 dik   Two-hybrid Drosophila melanogaster 27 Dap160   Two-hybrid Drosophila melanogaster 28 Nup54   Two-hybrid Drosophila melanogaster 29 Vps37B   Two-hybrid Drosophila melanogaster Two-hybrid Drosophila melanogaster Affinity Capture-MS Drosophila melanogaster 30 tum 36538 Two-hybrid Drosophila melanogaster 31 ImpE3   Two-hybrid Drosophila melanogaster 32 cactin   Two-hybrid Drosophila melanogaster 33 CG1597   Two-hybrid Drosophila melanogaster 34 CG7352   Two-hybrid Drosophila melanogaster 35 dom   Two-hybrid Drosophila melanogaster 36 qtc   Two-hybrid Drosophila melanogaster 37 Cip4   Two-hybrid Drosophila melanogaster 38 Leash   Affinity Capture-MS Drosophila melanogaster 39 Mf   Two-hybrid Drosophila melanogaster View the network

Pathways in which TSG101 is involved

No pathways found