Gene description for tum
Gene name tumbleweed
Gene symbol tum
Other names/aliases 13345
AcGAP
CG13345
DRacGAP
DmelCG13345
RacGAP
RacGAP50C
RacGAP50c
RacGap
RacGap50C
RhoGAP-50C14
TUM
acGAP
eon
i249
racGAP50
racGAP50C
racGAP50c
Species Drosophila melanogaster
 Database cross references - tum
ExoCarta ExoCarta_36538
Vesiclepedia VP_36538
Entrez Gene 36538
 tum identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for tum
Biological Process
    mitotic cytokinesis GO:0000281 IMP
    cell fate determination GO:0001709 IMP
    Rho protein signal transduction GO:0007266 IGI
    somatic muscle development GO:0007525 IMP
    larval somatic muscle development GO:0007526 IMP
    protein localization GO:0008104 IMP
    response to wounding GO:0009611 IMP
    regulation of cytokinesis GO:0032465 IMP
    maintenance of protein location in cell GO:0032507 IMP
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 IMP
    wound healing GO:0042060 IMP
    dendrite morphogenesis GO:0048813 IMP
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IMP
    regulation of microtubule cytoskeleton organization GO:0070507 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 IGI
    negative regulation of canonical Wnt signaling pathway GO:0090090 IMP
    regulation of stem cell differentiation GO:2000736 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    spindle GO:0005819 IDA
    midbody GO:0030496 IDA
    cleavage furrow GO:0032154 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    cortical microtubule GO:0055028 IDA
    centralspindlin complex GO:0097149 IDA
 Experiment description of studies that identified tum in exosomes
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for tum
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hira  
Affinity Capture-MS Drosophila melanogaster
2 CG32344  
Affinity Capture-MS Drosophila melanogaster
3 Caf1  
Affinity Capture-MS Drosophila melanogaster
4 TSG101 39881
Two-hybrid Drosophila melanogaster
5 pav 38515
Affinity Capture-MS Drosophila melanogaster
6 CG18748  
Affinity Capture-MS Drosophila melanogaster
View the network image/svg+xml
 Pathways in which tum is involved
No pathways found





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