Gene description for tum
Gene name tumbleweed
Gene symbol tum
Other names/aliases 13345
AcGAP
CG13345
DRacGAP
DmelCG13345
RacGAP
RacGAP50C
RacGAP50c
RacGap
RacGap50C
RhoGAP-50C14
TUM
acGAP
eon
i249
racGAP50
racGAP50C
racGAP50c
Species Drosophila melanogaster
 Database cross references - tum
ExoCarta ExoCarta_36538
Entrez Gene 36538
 tum identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for tum
Molecular Function
    GTPase activator activity GO:0005096 NAS
    diacylglycerol binding GO:0019992 ISS
    signal transducer activity GO:0004871 ISS
Biological Process
    negative regulation of Wnt signaling pathway GO:0030178 IMP
    regulation of Wnt signaling pathway GO:0030111 IMP
    regulation of microtubule cytoskeleton organization GO:0070507 IMP
    cell proliferation GO:0008283 IGI
    somatic muscle development GO:0007525 IMP
    neurogenesis GO:0022008 IMP
    cellular protein localization GO:0034613 IMP
    mitotic cytokinesis GO:0000281 IMP
    mitotic spindle assembly GO:0090307 IMP
    cell fate determination GO:0001709 IMP
    centrosome duplication GO:0051298 IMP
    neuron development GO:0048666 IMP
    dendrite morphogenesis GO:0048813 IMP
    regulation of cytokinesis GO:0032465 IMP
    maintenance of protein location in cell GO:0032507 IMP
    Rho protein signal transduction GO:0007266 IGI
    mitotic spindle organization GO:0007052 IMP
Subcellular Localization
    spindle GO:0005819 IDA
    nucleus GO:0005634 IDA
    cortical microtubule GO:0055028 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    midbody GO:0030496 IDA
 Experiment description of studies that identified tum in exosomes
1
Experiment ID 202
ISEV standards
IEM
EV Biophysical techniques
TSG10|RAB35
EV Cytosolic markers
FLOT2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for tum
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which tum is involved
No pathways found





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