Experiment description of studies that identified MEP1A in exosomes

1 Experiment ID 282 MISEV standards ✔ CEM EV Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM EV Enriched markers ✘ EV Negative markers ✔ DLS EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ. Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Ultracentrifugation - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

2 Experiment ID 283 MISEV standards ✔ CEM EV Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM EV Enriched markers ✘ EV Negative markers ✔ DLS EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ. Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Ultracentrifugation - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

3 Experiment ID 285 MISEV standards ✔ CEM EV Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM EV Enriched markers ✘ EV Negative markers ✔ DLS EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ. Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 Isolation/purification methods Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

4 Experiment ID 286 MISEV standards ✔ CEM EV Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM EV Enriched markers ✘ EV Negative markers ✔ DLS EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ. Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 Isolation/purification methods Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

5 Experiment ID 4 MISEV standards ✔ IEM EV Biophysical techniques ✔ CD63|MHCI|MHCII EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 11487543     Organism Homo sapiens Experiment description Intestinal epithelial cells secrete exosome-like vesicles. Authors van Niel G, Raposo G, Candalh C, Boussac M, Hershberg R, Cerf-Bensussan N, Heyman M Journal name Not applicable Publication year 2001 Sample Intestinal epithelial cells Sample name HT29-19A T84-DRB1*0401/CIITA Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [MALDI TOF] Western blotting

6 Experiment ID 488 MISEV standards ✔ EM EV Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for MEP1A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 GCTG   Biochemical Activity Homo sapiens 2 FN1 2335 Invitro Homo sapiens 3 MEP1A 4224 Invitro Homo sapiens Invitro Homo sapiens 4 GNRH1   Biochemical Activity Homo sapiens 5 NTS   Biochemical Activity Homo sapiens 6 BDKRB1 623 Biochemical Activity Homo sapiens 7 MEP1B 4225 Invitro Homo sapiens 8 PTH   Biochemical Activity Homo sapiens 9 LAMA1 284217 Biochemical Activity Homo sapiens 10 VIP   Biochemical Activity Homo sapiens 11 NPY   Biochemical Activity Homo sapiens 12 LAMA5 3911 Biochemical Activity Homo sapiens View the network

Pathways in which MEP1A is involved

No pathways found