Experiment ID |
283 |
MISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101|CD63|CD81|EpCAM
|
EV Enriched markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |