Gene description for LOC440434
Gene name aminopeptidase puromycin sensitive pseudogene
Gene symbol LOC440434
Other names/aliases -
Species Homo sapiens
 Database cross references - LOC440434
ExoCarta ExoCarta_440434
Vesiclepedia VP_440434
Entrez Gene 440434
 LOC440434 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for LOC440434
Molecular Function
    zinc ion binding GO:0008270 IBA
    peptide binding GO:0042277 IBA
    metalloaminopeptidase activity GO:0070006 IBA
Biological Process
    proteolysis GO:0006508 IBA
    peptide catabolic process GO:0043171 IBA
Subcellular Localization
    extracellular space GO:0005615 IBA
    cytoplasm GO:0005737 IBA
    membrane GO:0016020 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified LOC440434 in sEVs
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LOC440434
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PFN1 5216
Co-fractionation Homo sapiens
2 SERBP1 26135
Affinity Capture-MS Homo sapiens
3 NFKBIE  
Co-fractionation Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 LOC100132735  
Protein-RNA Homo sapiens
6 PRDX1 5052
Co-fractionation Homo sapiens
7 SORD 6652
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which LOC440434 is involved
No pathways found





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