Gene description for Vha36-1
Gene name Vacuolar H[+] ATPase 36kD subunit 1
Gene symbol Vha36-1
Other names/aliases ATP6V1D
CG8186
DmelCG8186
Vha36
VhaD
anon-WO0153538.20
anon-WO0153538.21
anon-WO0153538.28
anon-WO02059370.27
l(2)k07207
vha36
vha36-1
Species Drosophila melanogaster
 Database cross references - Vha36-1
ExoCarta ExoCarta_44702
Vesiclepedia VP_44702
Entrez Gene 44702
UniProt Q9V7D2  
 Vha36-1 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Vha36-1
Molecular Function
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IC
Biological Process
    proton transmembrane transport GO:1902600 IC
Subcellular Localization
    vacuolar proton-transporting V-type ATPase, V1 domain GO:0000221 ISS
    plasma membrane proton-transporting V-type ATPase complex GO:0033181 IMP
 Experiment description of studies that identified Vha36-1 in exosomes
1
Experiment ID 202
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG10|RAB35|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Vha36-1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CG8841  
Two-hybrid Drosophila melanogaster
2 Galphas  
Two-hybrid Drosophila melanogaster
3 Vha55  
Affinity Capture-MS Drosophila melanogaster
4 CG4623  
Two-hybrid Drosophila melanogaster
5 slim  
Affinity Capture-MS Drosophila melanogaster
6 Vha68-1  
Affinity Capture-MS Drosophila melanogaster
7 Vha26 40679
Affinity Capture-MS Drosophila melanogaster
8 atl  
Affinity Capture-MS Drosophila melanogaster
9 Vha44  
Affinity Capture-MS Drosophila melanogaster
10 VhaSFD  
Affinity Capture-MS Drosophila melanogaster
11 Vha68-2  
Affinity Capture-MS Drosophila melanogaster
12 CG33017  
Two-hybrid Drosophila melanogaster
13 Vha14-1  
Affinity Capture-MS Drosophila melanogaster
14 CG5126  
Affinity Capture-MS Drosophila melanogaster
15 CG12589  
Two-hybrid Drosophila melanogaster
16 CG14818  
Affinity Capture-MS Drosophila melanogaster
Two-hybrid Drosophila melanogaster
17 CG13510  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml



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