Gene description for Vha26
Gene name Vacuolar H[+]-ATPase 26kD subunit
Gene symbol Vha26
Other names/aliases ATP6V1E1
BcDNA.GH03683
BcDNA:GH03683
CG1088
DmelCG1088
VhaE
anon-83Bb
chr3R:1419698..1419836
vATPase
vha26
Species Drosophila melanogaster
 Database cross references - Vha26
ExoCarta ExoCarta_40679
Vesiclepedia VP_40679
Entrez Gene 40679
UniProt P54611  
 Vha26 identified in sEVs derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Vha26
Molecular Function
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IC
Biological Process
    terminal branching, open tracheal system GO:0007430 HMP
    terminal branching, open tracheal system GO:0007430 IMP
    liquid clearance, open tracheal system GO:0035002 HMP
    apical protein localization GO:0045176 IMP
    proton transmembrane transport GO:1902600 IC
Subcellular Localization
    vacuolar proton-transporting V-type ATPase, V1 domain GO:0000221 ISS
    plasma membrane GO:0005886 HDA
    plasma membrane proton-transporting V-type ATPase complex GO:0033181 IMP
 Experiment description of studies that identified Vha26 in sEVs
1
Experiment ID 202
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG10|RAB35|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Vha26
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vha55  
Affinity Capture-MS Drosophila melanogaster
2 26-29-p  
Two-hybrid Drosophila melanogaster
3 Vha13  
Two-hybrid Drosophila melanogaster
Two-hybrid Drosophila melanogaster
4 eff  
Two-hybrid Drosophila melanogaster
5 nwk  
Affinity Capture-MS Drosophila melanogaster
6 CG5126  
Affinity Capture-MS Drosophila melanogaster
7 Hmgs  
Affinity Capture-MS Drosophila melanogaster
8 Fmo-1  
Affinity Capture-MS Drosophila melanogaster
9 Ubi-p63E  
Two-hybrid Drosophila melanogaster
10 Vha68-1  
Affinity Capture-MS Drosophila melanogaster
11 CG8888  
Affinity Capture-MS Drosophila melanogaster
12 Vha68-2  
Affinity Capture-MS Drosophila melanogaster
13 vtd  
Two-hybrid Drosophila melanogaster
14 Tctp 41341
Two-hybrid Drosophila melanogaster
15 Tango2  
Two-hybrid Drosophila melanogaster
16 koi  
Two-hybrid Drosophila melanogaster
17 CG16826  
Two-hybrid Drosophila melanogaster
18 Vha36-1 44702
Affinity Capture-MS Drosophila melanogaster
19 Vha14-1  
Affinity Capture-MS Drosophila melanogaster
20 Cct2  
Affinity Capture-MS Drosophila melanogaster
21 Ef1alpha48D  
Two-hybrid Drosophila melanogaster
22 mip130  
Two-hybrid Drosophila melanogaster
23 Vha44  
Affinity Capture-MS Drosophila melanogaster
24 VhaSFD  
Two-hybrid Drosophila melanogaster
Affinity Capture-MS Drosophila melanogaster
25 Pk17E  
Two-hybrid Drosophila melanogaster
26 CG7277  
Affinity Capture-MS Drosophila melanogaster
27 CG32036  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml



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