Gene description for Aats-ile
Gene name Isoleucyl-tRNA synthetase
Gene symbol Aats-ile
Other names/aliases CG11471
DmelCG11471
IRS
IleRS
l(3)00827
Species Drosophila melanogaster
 Database cross references - Aats-ile
ExoCarta ExoCarta_45785
Vesiclepedia VP_45785
Entrez Gene 45785
 Aats-ile identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Aats-ile
Molecular Function
    isoleucine-tRNA ligase activity GO:0004822 TAS
Biological Process
    isoleucyl-tRNA aminoacylation GO:0006428 TAS
Subcellular Localization
    cytoplasm GO:0005737 TAS
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 IDA
 Experiment description of studies that identified Aats-ile in exosomes
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Aats-ile
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CG8235  
Affinity Capture-MS Drosophila melanogaster
2 Aats-glupro 42834
Affinity Capture-MS Drosophila melanogaster
3 Aats-arg  
Affinity Capture-MS Drosophila melanogaster
4 Aats-gln  
Affinity Capture-MS Drosophila melanogaster
5 Aats-asp  
Affinity Capture-MS Drosophila melanogaster
6 betaCOP  
Affinity Capture-MS Drosophila melanogaster
7 CG14894  
Proximity Label-MS Drosophila melanogaster
8 CG15100  
Affinity Capture-MS Drosophila melanogaster
View the network image/svg+xml
 Pathways in which Aats-ile is involved
No pathways found





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