Gene description for NCF4
Gene name neutrophil cytosolic factor 4, 40kDa
Gene symbol NCF4
Other names/aliases NCF
P40PHOX
SH3PXD4
Species Homo sapiens
 Database cross references - NCF4
ExoCarta ExoCarta_4689
Vesiclepedia VP_4689
Entrez Gene 4689
HGNC 7662
MIM 601488
UniProt Q15080  
 NCF4 identified in sEVs derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for NCF4
Molecular Function
    protein binding GO:0005515 IPI
    superoxide-generating NADPH oxidase activator activity GO:0016176 IBA
    superoxide-generating NADPH oxidase activator activity GO:0016176 IMP
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
Biological Process
    phagocytosis GO:0006909 IEA
    superoxide anion generation GO:0042554 IBA
    superoxide anion generation GO:0042554 IMP
    respiratory burst GO:0045730 NAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 NAS
    endosome membrane GO:0010008 IDA
    membrane GO:0016020 IDA
    phagolysosome GO:0032010 TAS
    NADPH oxidase complex GO:0043020 IBA
    NADPH oxidase complex GO:0043020 IDA
    NADPH oxidase complex GO:0043020 NAS
 Experiment description of studies that identified NCF4 in sEVs
1
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 XRCC6 2547
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 TXN 7295
Two-hybrid Homo sapiens
3 MSN 4478
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 NCF2 4688
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
5 NCF1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
6 PRKDC 5591
Biochemical Activity Homo sapiens
View the network image/svg+xml



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