Gene description for NCF4
Gene name neutrophil cytosolic factor 4, 40kDa
Gene symbol NCF4
Other names/aliases NCF
P40PHOX
SH3PXD4
Species Homo sapiens
 Database cross references - NCF4
ExoCarta ExoCarta_4689
Entrez Gene 4689
HGNC 7662
MIM 601488
UniProt Q15080  
 NCF4 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for NCF4
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
    superoxide-generating NADPH oxidase activator activity GO:0016176 IMP
    protein dimerization activity GO:0046983 TAS
Biological Process
    immune response GO:0006955 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent GO:0002479 TAS
    phagosome maturation GO:0090382 TAS
    oxidation-reduction process GO:0055114 TAS
    positive regulation of catalytic activity GO:0043085 IMP
    interaction with host GO:0051701 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class I GO:0042590 TAS
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
Subcellular Localization
    phagolysosome GO:0032010 TAS
    membrane GO:0016020 IDA
    cytosol GO:0005829 TAS
    endosome membrane GO:0010008 IDA
    NADPH oxidase complex GO:0043020 IDA
 Experiment description of studies that identified NCF4 in exosomes
1
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NCF2 4688
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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