Gene description for GPR143
Gene name G protein-coupled receptor 143
Gene symbol GPR143
Other names/aliases NYS6
OA1
Species Homo sapiens
 Database cross references - GPR143
ExoCarta ExoCarta_4935
Entrez Gene 4935
HGNC 20145
MIM 300808
UniProt P51810  
 GPR143 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for GPR143
Molecular Function
    G-protein coupled receptor activity GO:0004930 IDA
    dopamine binding GO:0035240 IDA
    tyrosine binding GO:0072545 IDA
    L-DOPA binding GO:0072544 IDA
    L-DOPA receptor activity GO:0035643 IDA
    protein binding GO:0005515 IPI
Biological Process
    calcium-mediated signaling using intracellular calcium source GO:0035584 IDA
    visual perception GO:0007601 TAS
    neuropeptide signaling pathway GO:0007218 IDA
    melanosome organization GO:0032438 IMP
    eye pigment biosynthetic process GO:0006726 TAS
    melanosome transport GO:0032402 IDA
    phosphatidylinositol-mediated signaling GO:0048015 IDA
    melanosome localization GO:0032400 ISS
    regulation of calcium-mediated signaling GO:0050848 IDA
    signal transduction GO:0007165 TAS
    G-protein coupled receptor signaling pathway GO:0007186 IMP
Subcellular Localization
    lysosomal membrane GO:0005765 IDA
    cytoplasm GO:0005737 TAS
    integral component of membrane GO:0016021 IEA
    membrane GO:0016020 TAS
    Golgi apparatus GO:0005794 IDA
    melanosome GO:0042470 IDA
    plasma membrane GO:0005886 IDA
    melanosome membrane GO:0033162 IDA
    apical plasma membrane GO:0016324 IDA
 Experiment description of studies that identified GPR143 in exosomes
1
Experiment ID 254
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 255
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 256
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 257
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GPR143
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SUCLG2 8801
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 GNAI1 2770
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which GPR143 is involved
No pathways found





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