Gene description for PCNA
Gene name proliferating cell nuclear antigen
Gene symbol PCNA
Other names/aliases -
Species Bos taurus
 Database cross references - PCNA
ExoCarta ExoCarta_515499
Vesiclepedia VP_515499
Entrez Gene 515499
UniProt Q3ZBW4  
 PCNA identified in sEVs derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for PCNA
Molecular Function
    DNA binding GO:0003677 IEA
    DNA polymerase processivity factor activity GO:0030337 IEA
Biological Process
    leading strand elongation GO:0006272 IEA
    regulation of DNA replication GO:0006275 IEA
    mismatch repair GO:0006298 IEA
    translesion synthesis GO:0019985 IEA
Subcellular Localization
    PCNA complex GO:0043626 IEA
 Experiment description of studies that identified PCNA in sEVs
1
Experiment ID 213
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description "Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis."
Authors "Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD."
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PCNA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK2  
Affinity Capture-Western Bos taurus
2 DHX9  
Affinity Capture-Western Bos taurus
3 RFC2  
Affinity Capture-Western Bos taurus
4 POLR2E  
Affinity Capture-Western Bos taurus
5 DNTT  
Affinity Capture-Western Bos taurus
6 CDK4  
Affinity Capture-Western Bos taurus
7 CDK5  
Affinity Capture-Western Bos taurus
8 PCNA 515499
Affinity Capture-Western Bos taurus
Affinity Capture-Western Bos taurus
9 POLR2D  
Affinity Capture-Western Bos taurus
10 TOP1  
Affinity Capture-Western Bos taurus
11 RPA1  
Affinity Capture-Western Bos taurus
12 RFC4  
Affinity Capture-Western Bos taurus
View the network image/svg+xml
 Pathways in which PCNA is involved
PathwayEvidenceSource
Base Excision Repair IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Chromosome Maintenance IEA Reactome
DNA Damage Bypass IEA Reactome
DNA Double-Strand Break Repair IEA Reactome
DNA Repair IEA Reactome
DNA Replication IEA Reactome
DNA strand elongation IEA Reactome
Dual Incision in GG-NER IEA Reactome
Dual incision in TC-NER IEA Reactome
E3 ubiquitin ligases ubiquitinate target proteins IEA Reactome
Extension of Telomeres IEA Reactome
Gap-filling DNA repair synthesis and ligation in GG-NER IEA Reactome
Gap-filling DNA repair synthesis and ligation in TC-NER IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
Global Genome Nucleotide Excision Repair (GG-NER) IEA Reactome
HDR through Homologous Recombination (HRR) IEA Reactome
HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) IEA Reactome
Homology Directed Repair IEA Reactome
Lagging Strand Synthesis IEA Reactome
Leading Strand Synthesis IEA Reactome
Metabolism of proteins IEA Reactome
Mismatch Repair IEA Reactome
Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) IEA Reactome
Nucleotide Excision Repair IEA Reactome
PCNA-Dependent Long Patch Base Excision Repair IEA Reactome
Polymerase switching IEA Reactome
Polymerase switching on the C-strand of the telomere IEA Reactome
Post-translational protein modification IEA Reactome
Processive synthesis on the C-strand of the telomere IEA Reactome
Processive synthesis on the lagging strand IEA Reactome
Protein ubiquitination IEA Reactome
Recognition of DNA damage by PCNA-containing replication complex IEA Reactome
Removal of the Flap Intermediate IEA Reactome
Removal of the Flap Intermediate from the C-strand IEA Reactome
Resolution of Abasic Sites (AP sites) IEA Reactome
Resolution of AP sites via the multiple-nucleotide patch replacement pathway IEA Reactome
RNA Polymerase II Transcription IEA Reactome
S Phase IEA Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation IEA Reactome
SUMOylation of DNA replication proteins IEA Reactome
Synthesis of DNA IEA Reactome
Telomere C-strand (Lagging Strand) Synthesis IEA Reactome
Telomere Maintenance IEA Reactome
Termination of translesion DNA synthesis IEA Reactome
TP53 Regulates Transcription of Cell Cycle Genes IEA Reactome
TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest IEA Reactome
Transcription-Coupled Nucleotide Excision Repair (TC-NER) IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome
Translesion Synthesis by POLH IEA Reactome
Translesion synthesis by POLI IEA Reactome
Translesion synthesis by POLK IEA Reactome
Translesion synthesis by REV1 IEA Reactome
Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template IEA Reactome





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