Gene description for Paf1
Gene name Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
Gene symbol Paf1
Other names/aliases 5730511K23Rik
AA675045
Species Mus musculus
 Database cross references - Paf1
ExoCarta ExoCarta_54624
Entrez Gene 54624
UniProt Q8K2T8  
 Paf1 identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
 Gene ontology annotations for Paf1
Molecular Function
    RNA polymerase II core binding GO:0000993 ISO
    protein binding GO:0005515 IPI
    chromatin binding GO:0003682 IDA
Biological Process
    histone H2B ubiquitination GO:0033523 ISO
    histone monoubiquitination GO:0010390 ISO
    transcription, DNA-templated GO:0006351 IEA
    Wnt signaling pathway GO:0016055 IEA
    mRNA polyadenylation GO:0006378 ISO
    negative regulation of myeloid cell differentiation GO:0045638 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    positive regulation of transcription elongation from RNA polymerase II promoter GO:0032968 ISO
    endodermal cell fate commitment GO:0001711 IMP
    cellular response to lipopolysaccharide GO:0071222 IDA
    regulation of transcription, DNA-templated GO:0006355 IEA
    protein localization to nucleus GO:0034504 ISO
    positive regulation of mRNA 3'-end processing GO:0031442 ISO
    stem cell maintenance GO:0019827 IMP
    positive regulation of histone methylation GO:0031062 ISO
    nucleosome positioning GO:0016584 ISO
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
Subcellular Localization
    Cdc73/Paf1 complex GO:0016593 ISO
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Paf1 in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Paf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Paf1 is involved
No pathways found





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