Gene description for CRLS1
Gene name cardiolipin synthase 1
Gene symbol CRLS1
Other names/aliases C20orf155
CLS
CLS1
GCD10
dJ967N21.6
Species Homo sapiens
 Database cross references - CRLS1
ExoCarta ExoCarta_54675
Vesiclepedia VP_54675
Entrez Gene 54675
HGNC 16148
MIM 608188
UniProt Q9UJA2  
 CRLS1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for CRLS1
Molecular Function
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 TAS
    cardiolipin synthase (CMP-forming) GO:0043337 IBA
    cardiolipin synthase (CMP-forming) GO:0043337 IDA
    cardiolipin synthase (CMP-forming) GO:0043337 IMP
    cardiolipin synthase (CMP-forming) GO:0043337 TAS
    2-acylglycerol-3-phosphate O-acyltransferase activity GO:0047144 TAS
Biological Process
    cardiolipin biosynthetic process GO:0032049 IBA
    cardiolipin biosynthetic process GO:0032049 IDA
    cardiolipin biosynthetic process GO:0032049 TAS
    phosphatidylglycerol acyl-chain remodeling GO:0036148 TAS
    response to thyroxine GO:0097068 IEA
    response to phosphatidylethanolamine GO:1905711 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial inner membrane GO:0005743 TAS
 Experiment description of studies that identified CRLS1 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for CRLS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EGFR 1956
Negative Genetic Homo sapiens
2 PINK1  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 F2RL1  
Two-hybrid Homo sapiens
4 FBXO15 201456
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
5 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
View the network image/svg+xml



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