Gene description for DNAH3
Gene name dynein, axonemal, heavy chain 3
Gene symbol DNAH3
Other names/aliases DLP3
DNAHC3B
HEL-36
Hsadhc3
dnahc3-b
Species Homo sapiens
 Database cross references - DNAH3
ExoCarta ExoCarta_55567
Vesiclepedia VP_55567
Entrez Gene 55567
HGNC 2949
MIM 603334
UniProt Q8TD57  
 DNAH3 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for DNAH3
Molecular Function
    microtubule motor activity GO:0003777 NAS
    ATP binding GO:0005524 IEA
    minus-end-directed microtubule motor activity GO:0008569 IBA
    ATP hydrolysis activity GO:0016887 IEA
    dynein intermediate chain binding GO:0045505 IBA
    dynein light intermediate chain binding GO:0051959 IBA
Biological Process
    cilium-dependent cell motility GO:0060285 NAS
    cilium movement involved in cell motility GO:0060294 IBA
Subcellular Localization
    axonemal dynein complex GO:0005858 NAS
    microtubule GO:0005874 IEA
    inner dynein arm GO:0036156 IBA
    9+2 motile cilium GO:0097729 IBA
 Experiment description of studies that identified DNAH3 in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for DNAH3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CANX 821
Cross-Linking-MS (XL-MS) Homo sapiens
2 RPL26 6154
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
3 MKI67  
Affinity Capture-MS Homo sapiens
4 ARRB1 408
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DNAH3 is involved
No pathways found





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