Gene description for Sfn
Gene name stratifin
Gene symbol Sfn
Other names/aliases Er
Mme1
Ywhas
Species Mus musculus
 Database cross references - Sfn
ExoCarta ExoCarta_55948
Entrez Gene 55948
UniProt O70456  
 Sfn identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Mast cells 17486113    
 Gene ontology annotations for Sfn
Molecular Function
    protein kinase binding GO:0019901 IDA
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IEA
    phosphoprotein binding GO:0051219 IPI
Biological Process
    keratinocyte differentiation GO:0030216 IMP
    positive regulation of protein export from nucleus GO:0046827 IDA
    skin development GO:0043588 IMP
    keratinization GO:0031424 IMP
    regulation of epidermal cell division GO:0010482 IMP
    positive regulation of epidermal cell differentiation GO:0045606 IMP
    keratinocyte development GO:0003334 IGI
    regulation of cell cycle GO:0051726 IDA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 ISO
    release of cytochrome c from mitochondria GO:0001836 ISO
    establishment of skin barrier GO:0061436 IMP
    positive regulation of cell growth GO:0030307 IDA
    regulation of cyclin-dependent protein serine/threonine kinase activity GO:0000079 IDA
    negative regulation of keratinocyte proliferation GO:0010839 IGI
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    extracellular region GO:0005576 IEA
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Sfn in exosomes
1
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Sfn
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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