Gene description for Rala
Gene name v-ral simian leukemia viral oncogene homolog A (ras related)
Gene symbol Rala
Other names/aliases 3010001O15Rik
AW322615
Ral
Rasl1
Species Mus musculus
 Database cross references - Rala
ExoCarta ExoCarta_56044
Vesiclepedia VP_56044
Entrez Gene 56044
UniProt P63321  
 Rala identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rala
Molecular Function
    nucleotide binding GO:0000166 IEA
    ATPase binding GO:0051117 ISO
    GDP binding GO:0019003 ISO
    myosin binding GO:0017022 ISO
    GTPase activity GO:0003924 IEA
    GTP binding GO:0005525 ISO
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 ISO
    Edg-2 lysophosphatidic acid receptor binding GO:0031755 ISO
Biological Process
    regulation of exocytosis GO:0017157 ISO
    actin cytoskeleton reorganization GO:0031532 ISO
    cytokinesis GO:0000910 ISO
    small GTPase mediated signal transduction GO:0007264 IEA
    Ras protein signal transduction GO:0007265 IEA
    exocytosis GO:0006887 IEA
    membrane raft localization GO:0051665 ISO
    cell cycle GO:0007049 IEA
    signal transduction GO:0007165 IEA
    positive regulation of filopodium assembly GO:0051491 ISO
    cell division GO:0051301 IEA
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 IDA
    cleavage furrow GO:0032154 ISO
    plasma membrane GO:0005886 ISO
    focal adhesion GO:0005925 ISO
    endocytic vesicle GO:0030139 ISO
    cell surface GO:0009986 ISO
    myelin sheath GO:0043209 IDA
 Experiment description of studies that identified Rala in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 39
MISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rala
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rala is involved
PathwayEvidenceSource
p38MAPK events IEA Reactome
Translocation of GLUT4 to the plasma membrane IEA Reactome





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