Gene description for Rap2b
Gene name RAP2B, member of RAS oncogene family
Gene symbol Rap2b
Other names/aliases 4021402C18Rik
AA408554
Species Mus musculus
 Database cross references - Rap2b
ExoCarta ExoCarta_74012
Vesiclepedia VP_74012
Entrez Gene 74012
UniProt P61226  
 Rap2b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rap2b
Molecular Function
    GTPase activity GO:0003924 IBA
    G protein activity GO:0003925 IEA
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GDP binding GO:0019003 IBA
    GDP binding GO:0019003 IEA
    GDP binding GO:0019003 ISO
    protein domain specific binding GO:0019904 IPI
Biological Process
    platelet activation GO:0030168 ISO
    negative regulation of cell migration GO:0030336 IBA
    negative regulation of cell migration GO:0030336 IDA
    positive regulation of protein autophosphorylation GO:0031954 IDA
    Rap protein signal transduction GO:0032486 IBA
    Rap protein signal transduction GO:0032486 IDA
    regulation of protein tyrosine kinase activity GO:0061097 IDA
    platelet aggregation GO:0070527 IEA
    platelet aggregation GO:0070527 ISO
Subcellular Localization
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    bicellular tight junction GO:0005923 IEA
    bicellular tight junction GO:0005923 ISO
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    cell-cell contact zone GO:0044291 IEA
    cell-cell contact zone GO:0044291 ISO
    membrane raft GO:0045121 IEA
    membrane raft GO:0045121 ISO
    recycling endosome GO:0055037 IDA
    recycling endosome membrane GO:0055038 IDA
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Rap2b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rap2b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Tsc1  
Affinity Capture-MS Mus musculus
3 Sstr3  
Proximity Label-MS Mus musculus
4 Rala 56044
Co-fractionation Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
7 Mapt  
Affinity Capture-MS Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Rap2b is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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