Gene description for Krt71
Gene name keratin 71
Gene symbol Krt71
Other names/aliases AA589543
Ca
Cal4
Cu
Krt2-6g
mK6irs
mK6irs1
Species Mus musculus
 Database cross references - Krt71
ExoCarta ExoCarta_56735
Vesiclepedia VP_56735
Entrez Gene 56735
UniProt Q9R0H5  
 Krt71 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Oligodendrocytes 21136642    
 Gene ontology annotations for Krt71
Molecular Function
    structural constituent of cytoskeleton GO:0005200 TAS
    structural constituent of skin epidermis GO:0030280 IBA
Biological Process
    cytoskeleton organization GO:0007010 TAS
    hair follicle morphogenesis GO:0031069 IMP
    hair follicle morphogenesis GO:0031069 ISO
    hair follicle morphogenesis GO:0031069 ISS
    keratinization GO:0031424 IBA
    intermediate filament organization GO:0045109 IBA
    intermediate filament organization GO:0045109 ISO
    intermediate filament organization GO:0045109 ISS
Subcellular Localization
    cytoplasm GO:0005737 IEA
    intermediate filament GO:0005882 TAS
    keratin filament GO:0045095 IBA
    keratin filament GO:0045095 IDA
    keratin filament GO:0045095 ISO
 Experiment description of studies that identified Krt71 in exosomes
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Krt71
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITSN1 6453
Two-hybrid Homo sapiens
2 Krt27  
Co-fractionation Mus musculus
3 Krt40  
Co-fractionation Mus musculus
4 Krt13 16663
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Krt71 is involved
PathwayEvidenceSource
Developmental Biology IEA Reactome
Formation of the cornified envelope IEA Reactome
Keratinization IEA Reactome





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