Gene description for SPHK2
Gene name sphingosine kinase 2
Gene symbol SPHK2
Other names/aliases SK 2
SK-2
SPK 2
SPK-2
Species Homo sapiens
 Database cross references - SPHK2
ExoCarta ExoCarta_56848
Vesiclepedia VP_56848
Entrez Gene 56848
HGNC 18859
MIM 607092
UniProt Q9NRA0  
 SPHK2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SPHK2
Molecular Function
    lipid kinase activity GO:0001727 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    sphingosine kinase activity GO:0008481 IDA
    D-erythro-sphingosine kinase activity GO:0017050 IBA
    D-erythro-sphingosine kinase activity GO:0017050 IDA
    small GTPase binding GO:0031267 NAS
    sphingosine-1-phosphate receptor activity GO:0038036 IMP
    histone binding GO:0042393 IDA
Biological Process
    blood vessel development GO:0001568 IEA
    positive regulation of cytokine production involved in immune response GO:0002720 ISS
    sphingosine-1-phosphate receptor signaling pathway GO:0003376 IEA
    sphinganine-1-phosphate biosynthetic process GO:0006669 IBA
    sphinganine-1-phosphate biosynthetic process GO:0006669 IDA
    sphingosine metabolic process GO:0006670 ISS
    brain development GO:0007420 IEA
    female pregnancy GO:0007565 IEA
    cell population proliferation GO:0008283 IEA
    positive regulation of cell population proliferation GO:0008284 IEA
    sphingolipid biosynthetic process GO:0030148 TAS
    negative regulation of cell growth GO:0030308 ISS
    positive regulation of interleukin-13 production GO:0032736 ISS
    positive regulation of interleukin-6 production GO:0032755 ISS
    positive regulation of tumor necrosis factor production GO:0032760 ISS
    positive regulation of mast cell activation involved in immune response GO:0033008 ISS
    intracellular signal transduction GO:0035556 ISS
    positive regulation of apoptotic process GO:0043065 IBA
    positive regulation of apoptotic process GO:0043065 IDA
    regulation of canonical NF-kappaB signal transduction GO:0043122 ISS
    positive regulation of mast cell degranulation GO:0043306 ISS
    transcription initiation-coupled chromatin remodeling GO:0045815 IDA
    sphingosine biosynthetic process GO:0046512 IBA
    sphingosine biosynthetic process GO:0046512 IGI
    sphingosine biosynthetic process GO:0046512 IMP
    regulation of reactive oxygen species biosynthetic process GO:1903426 ISS
    cellular response to phorbol 13-acetate 12-myristate GO:1904628 IDA
    regulation of cytochrome-c oxidase activity GO:1904959 IMP
    positive regulation of ceramide biosynthetic process GO:2000304 IDA
    regulation of ATP biosynthetic process GO:2001169 ISS
Subcellular Localization
    nucleosome GO:0000786 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 ISS
    mitochondrial inner membrane GO:0005743 IEA
    lysosomal membrane GO:0005765 HDA
    endoplasmic reticulum GO:0005783 ISS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    intracellular membrane-bounded organelle GO:0043231 IBA
 Experiment description of studies that identified SPHK2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SPHK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB9A 9367
Affinity Capture-MS Homo sapiens
2 C2CD4A  
Affinity Capture-MS Homo sapiens
3 RPL15 6138
Affinity Capture-MS Homo sapiens
4 NEUROG3  
Affinity Capture-MS Homo sapiens
5 HECW2  
Affinity Capture-MS Homo sapiens
6 HIST1H3A 8350
Affinity Capture-Western Homo sapiens
7 NEDD4L 23327
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 DUXAP10  
Affinity Capture-MS Homo sapiens
9 DDX58 23586
Affinity Capture-RNA Homo sapiens
View the network image/svg+xml
 Pathways in which SPHK2 is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Sphingolipid de novo biosynthesis TAS Reactome
Sphingolipid metabolism TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here