Gene description for Ehd3
Gene name EH-domain containing 3
Gene symbol Ehd3
Other names/aliases Ehd2
Species Mus musculus
 Database cross references - Ehd3
ExoCarta ExoCarta_57440
Vesiclepedia VP_57440
Entrez Gene 57440
UniProt Q9QXY6  
 Ehd3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ehd3
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    GTP binding GO:0005525 IEA
    lipid binding GO:0008289 IEA
Biological Process
    receptor recycling GO:0001881 IEA
    receptor recycling GO:0001881 ISO
    endocytosis GO:0006897 IBA
    protein transport GO:0015031 IEA
    endocytic recycling GO:0032456 IBA
    endocytic recycling GO:0032456 ISO
    endocytic recycling GO:0032456 ISS
    early endosome to Golgi transport GO:0034498 IEA
    early endosome to Golgi transport GO:0034498 ISO
    protein homooligomerization GO:0051260 ISO
    protein homooligomerization GO:0051260 ISS
    regulation of cardiac muscle contraction GO:0055117 IMP
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 IMP
    cilium assembly GO:0060271 ISO
    cilium assembly GO:0060271 ISS
    protein localization to plasma membrane GO:0072659 IBA
    protein localization to plasma membrane GO:0072659 IMP
    protein localization to plasma membrane GO:0072659 ISO
    regulation of cardiac muscle cell membrane potential GO:0086036 IMP
    regulation of cardiac muscle cell membrane potential GO:0086036 ISO
    Golgi to lysosome transport GO:0090160 IEA
    Golgi to lysosome transport GO:0090160 ISO
    positive regulation of voltage-gated calcium channel activity GO:1901387 IMP
    regulation of Golgi organization GO:1903358 IEA
    regulation of Golgi organization GO:1903358 ISO
    regulation of cardiac conduction GO:1903779 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    early endosome GO:0005769 IBA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IBA
    ciliary pocket membrane GO:0020018 ISO
    ciliary pocket membrane GO:0020018 ISS
    endocytic vesicle GO:0030139 IBA
    endocytic vesicle GO:0030139 IDA
    myelin sheath GO:0043209 HDA
    perinuclear region of cytoplasm GO:0048471 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
    recycling endosome membrane GO:0055038 IBA
    recycling endosome membrane GO:0055038 ISO
    recycling endosome membrane GO:0055038 ISS
 Experiment description of studies that identified Ehd3 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ehd3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sstr3  
Proximity Label-MS Mus musculus
2 Fmr1  
Affinity Capture-MS Mus musculus
3 Kcna3  
Affinity Capture-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Ehd3 57440
Two-hybrid Mus musculus
Two-hybrid Mus musculus
6 EHD1 10938
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which Ehd3 is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production IEA Reactome
Hemostasis IEA Reactome





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