Gene description for SHROOM4
Gene name shroom family member 4
Gene symbol SHROOM4
Other names/aliases SHAP
Species Homo sapiens
 Database cross references - SHROOM4
ExoCarta ExoCarta_57477
Vesiclepedia VP_57477
Entrez Gene 57477
HGNC 29215
MIM 300579
UniProt Q9ULL8  
 SHROOM4 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for SHROOM4
Molecular Function
    myosin II binding GO:0045159 ISS
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 ISS
Biological Process
    cell morphogenesis GO:0000902 IDA
    actin filament organization GO:0007015 IBA
    actin filament organization GO:0007015 ISS
    brain development GO:0007420 IDA
    actin cytoskeleton organization GO:0030036 NAS
    cognition GO:0050890 IDA
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IEA
Subcellular Localization
    stress fiber GO:0001725 ISS
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 ISS
    actin filament GO:0005884 IDA
    adherens junction GO:0005912 IBA
    focal adhesion GO:0005925 IDA
    cytoplasmic side of plasma membrane GO:0009898 IDA
    basal plasma membrane GO:0009925 ISS
    actin cytoskeleton GO:0015629 ISS
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 ISS
    cortical actin cytoskeleton GO:0030864 IBA
    cortical actin cytoskeleton GO:0030864 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
    apical junction complex GO:0043296 IBA
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
    GABA-ergic synapse GO:0098982 IEA
 Experiment description of studies that identified SHROOM4 in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for SHROOM4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FBXW7  
Synthetic Lethality Homo sapiens
2 DDX58 23586
Affinity Capture-RNA Homo sapiens
View the network image/svg+xml
 Pathways in which SHROOM4 is involved
No pathways found





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