Gene description for SHROOM4
Gene name shroom family member 4
Gene symbol SHROOM4
Other names/aliases SHAP
Species Homo sapiens
 Database cross references - SHROOM4
ExoCarta ExoCarta_57477
Entrez Gene 57477
HGNC 29215
MIM 300579
UniProt Q9ULL8  
 SHROOM4 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for SHROOM4
Molecular Function
    actin filament binding GO:0051015 ISS
Biological Process
    cell morphogenesis GO:0000902 IDA
    brain development GO:0007420 IDA
    actin cytoskeleton organization GO:0030036 NAS
    cognition GO:0050890 IDA
    actin filament organization GO:0007015 ISS
Subcellular Localization
    stress fiber GO:0001725 ISS
    filamentous actin GO:0031941 IDA
    basal plasma membrane GO:0009925 ISS
    myosin II complex GO:0016460 ISS
    actin cytoskeleton GO:0015629 ISS
    cytoplasmic side of plasma membrane GO:0009898 IDA
    cytoplasm GO:0005737 ISS
    apical plasma membrane GO:0016324 ISS
    cortical actin cytoskeleton GO:0030864 ISS
 Experiment description of studies that identified SHROOM4 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for SHROOM4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SHROOM4 is involved
No pathways found





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