Gene description for Stx6
Gene name syntaxin 6
Gene symbol Stx6
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Stx6
ExoCarta ExoCarta_60562
Vesiclepedia VP_60562
Entrez Gene 60562
UniProt Q63635  
 Stx6 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Stx6
Molecular Function
    SNARE binding GO:0000149 IBA
    SNAP receptor activity GO:0005484 IBA
    SNAP receptor activity GO:0005484 IEA
    protein binding GO:0005515 IPI
    syntaxin binding GO:0019905 ISO
Biological Process
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 IEA
    vesicle fusion GO:0006906 IBA
    vesicle fusion GO:0006906 ISO
    endosome organization GO:0007032 ISO
    synaptic vesicle to endosome fusion GO:0016189 IDA
    synaptic vesicle to endosome fusion GO:0016189 IMP
    vesicle-mediated transport GO:0016192 IDA
    endocytic recycling GO:0032456 IDA
    endocytic recycling GO:0032456 ISO
    endocytic recycling GO:0032456 ISO
    regulation of protein localization GO:0032880 ISO
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IDA
    retrograde transport, endosome to Golgi GO:0042147 ISO
    Golgi vesicle transport GO:0048193 IEA
    vesicle docking GO:0048278 IBA
    membrane fusion GO:0061025 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    early endosome GO:0005769 ISO
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 IEA
    trans-Golgi network GO:0005802 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 ISO
    synaptic vesicle membrane GO:0030672 EXP
    synaptic vesicle membrane GO:0030672 IBA
    synaptic vesicle membrane GO:0030672 IDA
    SNARE complex GO:0031201 IBA
    SNARE complex GO:0031201 ISO
    trans-Golgi network membrane GO:0032588 IDA
    trans-Golgi network membrane GO:0032588 ISO
    terminal bouton GO:0043195 HDA
    phagocytic vesicle GO:0045335 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    recycling endosome GO:0055037 IDA
    recycling endosome membrane GO:0055038 IEA
 Experiment description of studies that identified Stx6 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Stx6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GOPC 57120
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 March2  
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Co-localization Rattus norvegicus
View the network image/svg+xml



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