Gene description for Calr
Gene name calreticulin
Gene symbol Calr
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Calr
ExoCarta ExoCarta_64202
Entrez Gene 64202
UniProt P18418  
 Calr identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Calr
Molecular Function
    peptide binding GO:0042277 IDA
    poly(A) RNA binding GO:0044822 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    integrin binding GO:0005178 ISO
    calcium ion binding GO:0005509 ISO
    hormone binding GO:0042562 IPI
    androgen receptor binding GO:0050681 ISO
    mRNA binding GO:0003729 ISO
    iron ion binding GO:0005506 IDA
    carbohydrate binding GO:0030246 ISO
    protein binding GO:0005515 IPI
    unfolded protein binding GO:0051082 IMP
    glycoprotein binding GO:0001948 ISO
Biological Process
    response to testosterone GO:0033574 IEP
    cellular response to organic substance GO:0071310 IEP
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISO
    positive regulation of DNA replication GO:0045740 ISO
    cellular senescence GO:0090398 ISO
    positive regulation of phagocytosis GO:0050766 ISO
    positive regulation of dendritic cell chemotaxis GO:2000510 ISO
    regulation of meiotic nuclear division GO:0040020 ISO
    protein folding GO:0006457 TAS
    peptide antigen assembly with MHC class I protein complex GO:0002502 ISO
    cellular response to lithium ion GO:0071285 IEP
    negative regulation of neuron differentiation GO:0045665 ISO
    negative regulation of transcription, DNA-templated GO:0045892 ISO
    negative regulation of intracellular steroid hormone receptor signaling pathway GO:0033144 ISO
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    negative regulation of translation GO:0017148 IDA
    cell cycle arrest GO:0007050 ISO
    chaperone-mediated protein folding GO:0061077 IMP
    protein localization to nucleus GO:0034504 ISO
    positive regulation of cell proliferation GO:0008284 ISO
    response to organic substance GO:0010033 IEP
    protein stabilization GO:0050821 ISS
    response to estradiol GO:0032355 IEP
    protein export from nucleus GO:0006611 ISO
    spermatogenesis GO:0007283 IEP
    cortical actin cytoskeleton organization GO:0030866 ISO
    cardiac muscle cell differentiation GO:0055007 IEP
    positive regulation of cell cycle GO:0045787 ISO
    response to drug GO:0042493 IEP
    negative regulation of retinoic acid receptor signaling pathway GO:0048387 ISO
Subcellular Localization
    membrane GO:0016020 ISO
    external side of plasma membrane GO:0009897 ISO
    cell surface GO:0009986 IDA
    nucleus GO:0005634 ISO
    acrosomal vesicle GO:0001669 IDA
    focal adhesion GO:0005925 ISO
    cytosol GO:0005829 ISO
    cytoplasm GO:0005737 ISO
    intracellular membrane-bounded organelle GO:0043231 IDA
    smooth endoplasmic reticulum GO:0005790 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    extracellular exosome GO:0070062 ISO
    polysome GO:0005844 ISO
    protein complex GO:0043234 IDA
    endoplasmic reticulum lumen GO:0005788 ISO
    extracellular matrix GO:0031012 IDA
    sarcoplasmic reticulum GO:0016529 IDA
    extracellular space GO:0005615 ISO
    MHC class I peptide loading complex GO:0042824 ISO
    Golgi apparatus GO:0005794 IDA
    endoplasmic reticulum GO:0005783 TAS
    sarcoplasmic reticulum lumen GO:0033018 IEA
 Experiment description of studies that identified Calr in exosomes
1
Experiment ID 225
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD81|CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 90
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70|HSP90|TSG101
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 94
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 95
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 98
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Calr
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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