Gene description for Myl12b
Gene name myosin, light chain 12B, regulatory
Gene symbol Myl12b
Other names/aliases 1500001M02Rik
C77744
Mylc2b
RLC-B
Species Mus musculus
 Database cross references - Myl12b
ExoCarta ExoCarta_67938
Vesiclepedia VP_67938
Entrez Gene 67938
UniProt Q3THE2  
 Myl12b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Myl12b
Molecular Function
    calcium ion binding GO:0005509 IEA
    myosin heavy chain binding GO:0032036 IBA
    myosin heavy chain binding GO:0032036 IPI
Biological Process
    regulation of cell shape GO:0008360 IMP
Subcellular Localization
    stress fiber GO:0001725 IBA
    stress fiber GO:0001725 IDA
    cytoplasm GO:0005737 IBA
    brush border GO:0005903 IDA
    cell cortex GO:0005938 ISO
    cell cortex GO:0005938 ISS
    myosin II complex GO:0016460 IBA
    myosin II complex GO:0016460 IDA
    myofibril GO:0030016 IBA
    Z disc GO:0030018 IDA
    protein-containing complex GO:0032991 ISO
    apical part of cell GO:0045177 IDA
 Experiment description of studies that identified Myl12b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Myl12b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 Nphp4  
Affinity Capture-MS Mus musculus
4 SNCA 6622
Affinity Capture-MS Homo sapiens
5 Atxn1  
Affinity Capture-MS Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Tmem173  
Proximity Label-MS Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
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