Gene description for Ndufb4
Gene name NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4
Gene symbol Ndufb4
Other names/aliases 0610006N12Rik
1300010H20Rik
Species Mus musculus
 Database cross references - Ndufb4
ExoCarta ExoCarta_68194
Vesiclepedia VP_68194
Entrez Gene 68194
UniProt Q9CQC7  
 Ndufb4 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ndufb4
Molecular Function
    molecular_function GO:0003674 ND
Biological Process
    response to oxidative stress GO:0006979 ISO
    aerobic respiration GO:0009060 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 ISO
    mitochondrial respiratory chain complex I assembly GO:0032981 ISS
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 ISO
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 ISO
    respiratory chain complex I GO:0045271 ISS
 Experiment description of studies that identified Ndufb4 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ndufb4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Uqcrh  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
2 Ndufb10  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
3 SNCA 6622
Affinity Capture-MS Homo sapiens
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Ndufb5  
Co-fractionation Mus musculus
6 Mapt  
Affinity Capture-MS Mus musculus
7 Ndufb8  
Cross-Linking-MS (XL-MS) Mus musculus
8 Ndufb9  
Cross-Linking-MS (XL-MS) Mus musculus
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