Gene description for Pxdn
Gene name peroxidasin homolog (Drosophila)
Gene symbol Pxdn
Other names/aliases 2310075M15Rik
C85409
E330004E07
VPO1
mKIAA0230
Species Mus musculus
 Database cross references - Pxdn
ExoCarta ExoCarta_69675
Vesiclepedia VP_69675
Entrez Gene 69675
UniProt Q3UQ28  
 Pxdn identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pxdn
Molecular Function
    peroxidase activity GO:0004601 IBA
    peroxidase activity GO:0004601 ISO
    peroxidase activity GO:0004601 ISS
    extracellular matrix structural constituent GO:0005201 ISO
    extracellular matrix structural constituent GO:0005201 RCA
    oxidoreductase activity, acting on peroxide as acceptor GO:0016684 IDA
    oxidoreductase activity, acting on peroxide as acceptor GO:0016684 IMP
    oxidoreductase activity, acting on peroxide as acceptor GO:0016684 ISO
    heme binding GO:0020037 ISO
    heme binding GO:0020037 ISS
    laminin-1 binding GO:0043237 ISO
    laminin-1 binding GO:0043237 ISS
    metal ion binding GO:0046872 IEA
    lactoperoxidase activity GO:0140825 IEA
Biological Process
    angiogenesis GO:0001525 ISO
    angiogenesis GO:0001525 ISS
    eye development GO:0001654 IMP
    response to oxidative stress GO:0006979 IEA
    cell adhesion GO:0007155 IMP
    extracellular matrix organization GO:0030198 IDA
    extracellular matrix organization GO:0030198 ISO
    collagen fibril organization GO:0030199 IEA
    collagen fibril organization GO:0030199 ISO
    hydrogen peroxide catabolic process GO:0042744 ISO
    hydrogen peroxide catabolic process GO:0042744 ISS
    protein homooligomerization GO:0051260 ISO
    protein homooligomerization GO:0051260 ISS
    protein homotrimerization GO:0070207 ISO
    protein homotrimerization GO:0070207 ISS
    basement membrane assembly GO:0070831 IDA
    basement membrane assembly GO:0070831 IMP
    basement membrane assembly GO:0070831 ISO
    basement membrane assembly GO:0070831 ISO
    basement membrane organization GO:0071711 IMP
    cellular oxidant detoxification GO:0098869 IEA
Subcellular Localization
    basement membrane GO:0005604 IDA
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISS
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum GO:0005783 ISS
    cell surface GO:0009986 ISO
    cell surface GO:0009986 ISS
    extracellular matrix GO:0031012 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IDA
    collagen-containing extracellular matrix GO:0062023 ISO
 Experiment description of studies that identified Pxdn in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pxdn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Col5a2  
Co-fractionation Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Col5a1 12831
Co-fractionation Mus musculus
View the network image/svg+xml



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