Gene description for Copb1
Gene name coatomer protein complex, subunit beta 1
Gene symbol Copb1
Other names/aliases 2610019B04Rik
Species Mus musculus
 Database cross references - Copb1
ExoCarta ExoCarta_70349
Vesiclepedia VP_70349
Entrez Gene 70349
UniProt Q9JIF7  
 Copb1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Copb1
Molecular Function
    structural molecule activity GO:0005198 IEA
Biological Process
    intracellular protein transport GO:0006886 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    intra-Golgi vesicle-mediated transport GO:0006891 IEA
    intra-Golgi vesicle-mediated transport GO:0006891 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 ISO
    Golgi-associated vesicle GO:0005798 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    COPI vesicle coat GO:0030126 IBA
    COPI vesicle coat GO:0030126 IEA
    COPI vesicle coat GO:0030126 ISO
    COPI-coated vesicle GO:0030137 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Copb1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Copb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cope  
Co-fractionation Mus musculus
2 Sacm1l 83493
Co-fractionation Mus musculus
3 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
4 Foxp3  
Affinity Capture-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Arfgap2  
Co-fractionation Mus musculus
7 Copz1  
Co-fractionation Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
9 Arf3 11842
Co-fractionation Mus musculus
10 Pou5f1  
Affinity Capture-MS Mus musculus
11 ABCG1  
Affinity Capture-MS Homo sapiens
12 Nudcd1  
Co-fractionation Mus musculus
13 Fancd2  
Affinity Capture-MS Mus musculus
14 Kcna3  
Affinity Capture-MS Mus musculus
15 Copb2 50797
Co-fractionation Mus musculus
16 Eif5b 226982
Co-fractionation Mus musculus
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