Gene description for Sacm1l
Gene name SAC1 (suppressor of actin mutations 1, homolog)-like (S. cerevisiae)
Gene symbol Sacm1l
Other names/aliases SAC1
Sac1p
mKIAA0851
Species Mus musculus
 Database cross references - Sacm1l
ExoCarta ExoCarta_83493
Vesiclepedia VP_83493
Entrez Gene 83493
UniProt Q9EP69  
 Sacm1l identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sacm1l
Molecular Function
    phosphatidylinositol-3-phosphate phosphatase activity GO:0004438 IEA
    phosphatidylinositol-3-phosphate phosphatase activity GO:0004438 ISO
    protein binding GO:0005515 IPI
    phosphatase activity GO:0016791 ISO
    phosphatase activity GO:0016791 ISS
    phosphatidylinositol-4-phosphate phosphatase activity GO:0043812 IBA
    phosphatidylinositol-4-phosphate phosphatase activity GO:0043812 ISO
    phosphatidylinositol-4-phosphate phosphatase activity GO:0043812 ISS
    phosphatidylinositol-3,5-bisphosphate phosphatase activity GO:0106018 ISO
Biological Process
    phosphatidylinositol dephosphorylation GO:0046856 IBA
    phosphatidylinositol dephosphorylation GO:0046856 ISO
    phosphatidylinositol dephosphorylation GO:0046856 ISS
    exocytic insertion of neurotransmitter receptor to postsynaptic membrane GO:0098967 IDA
    exocytic insertion of neurotransmitter receptor to postsynaptic membrane GO:0098967 IMP
    neurotransmitter receptor transport to postsynaptic membrane GO:0098969 ISO
    vesicle-mediated transport in synapse GO:0099003 IDA
    vesicle-mediated transport in synapse GO:0099003 IMP
    vesicle-mediated transport in synapse GO:0099003 ISO
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    Golgi membrane GO:0000139 ISS
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    AMPA glutamate receptor complex GO:0032281 IDA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
    endoplasmic reticulum-plasma membrane contact site GO:0140268 ISO
    endoplasmic reticulum-plasma membrane contact site GO:0140268 ISS
 Experiment description of studies that identified Sacm1l in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sacm1l
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COPG2 26958
Affinity Capture-MS Homo sapiens
2 COPZ1 22818
Affinity Capture-MS Homo sapiens
3 CLPX 10845
Affinity Capture-MS Homo sapiens
4 SMC4 10051
Affinity Capture-MS Homo sapiens
5 Copb1 70349
Co-fractionation Mus musculus
6 EFR3A 23167
Affinity Capture-MS Homo sapiens
7 Tmem173  
Proximity Label-MS Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
9 ARCN1 372
Affinity Capture-MS Homo sapiens
10 KLHDC2  
Affinity Capture-MS Homo sapiens
11 COPE 11316
Affinity Capture-MS Homo sapiens
12 COPA 1314
Affinity Capture-MS Homo sapiens
13 COPG1 22820
Affinity Capture-MS Homo sapiens
14 COPB1 1315
Affinity Capture-MS Homo sapiens
15 LCOR 84458
Affinity Capture-MS Homo sapiens
16 COPB2 9276
Affinity Capture-MS Homo sapiens
17 Copb2 50797
Co-fractionation Mus musculus
18 RPN1 6184
Affinity Capture-MS Homo sapiens
19 Copa 12847
Co-fractionation Mus musculus
20 DDOST 1650
Affinity Capture-MS Homo sapiens
21 ATP5B 506
Affinity Capture-MS Homo sapiens
22 Fancd2  
Affinity Capture-MS Mus musculus
23 CDC5L 988
Affinity Capture-MS Homo sapiens
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