Gene description for Copa
Gene name coatomer protein complex subunit alpha
Gene symbol Copa
Other names/aliases AU040324
xenin
Species Mus musculus
 Database cross references - Copa
ExoCarta ExoCarta_12847
Vesiclepedia VP_12847
Entrez Gene 12847
UniProt Q8CIE6  
 Copa identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Copa
Molecular Function
    mRNA binding GO:0003729 IDA
    hormone activity GO:0005179 IEA
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
Biological Process
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IEA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IBA
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    signal transduction GO:0007165 IEA
    pancreatic juice secretion GO:0030157 ISO
    protein localization to axon GO:0099612 IMP
    protein localization to cell leading edge GO:1902463 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 ISO
    COPI vesicle coat GO:0030126 IBA
    COPI vesicle coat GO:0030126 ISO
    COPI-coated vesicle GO:0030137 IDA
    axon GO:0030424 ISO
    growth cone GO:0030426 IDA
    growth cone GO:0030426 ISO
    neuronal cell body GO:0043025 ISO
 Experiment description of studies that identified Copa in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Copa
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Selo  
Co-fractionation Mus musculus
2 COPG2 26958
Affinity Capture-MS Homo sapiens
3 COPZ1 22818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 Stau1  
Co-fractionation Mus musculus
5 Spg20  
Co-fractionation Mus musculus
6 KCTD10 83892
Affinity Capture-MS Homo sapiens
7 TNFAIP1 7126
Affinity Capture-MS Homo sapiens
8 ATL2 64225
Affinity Capture-MS Homo sapiens
9 Tmem173  
Proximity Label-MS Mus musculus
10 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
11 Kcna3  
Affinity Capture-MS Mus musculus
12 Kctd13  
Affinity Capture-MS Mus musculus
13 BCAP31 10134
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ARCN1 372
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TMEM263 90488
Affinity Capture-MS Homo sapiens
16 COPE 11316
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 COPA 1314
Affinity Capture-MS Homo sapiens
18 COPG1 22820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 Mettl21e  
Affinity Capture-MS Mus musculus
20 COPB1 1315
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 LZTS2 84445
Affinity Capture-MS Homo sapiens
22 ERGIC2 51290
Affinity Capture-MS Homo sapiens
23 Arfgap2  
Co-fractionation Mus musculus
24 Cnot1  
Co-fractionation Mus musculus
25 UBR4 23352
Affinity Capture-MS Homo sapiens
26 Unk  
Affinity Capture-RNA Mus musculus
27 COPB2 9276
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 Sacm1l 83493
Co-fractionation Mus musculus
29 N4BP3  
Affinity Capture-MS Homo sapiens
30 Slc25a11  
Co-fractionation Mus musculus
31 Eed  
Affinity Capture-MS Mus musculus
32 Atg16l1  
Affinity Capture-MS Mus musculus
33 Fancd2  
Affinity Capture-MS Mus musculus
34 SIPA1L3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 BCAP29 55973
Affinity Capture-MS Homo sapiens
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