Gene description for Cul2
Gene name cullin 2
Gene symbol Cul2
Other names/aliases 1300003D18Rik
4932411N15Rik
AI327301
mKIAA4106
Species Mus musculus
 Database cross references - Cul2
ExoCarta ExoCarta_71745
Vesiclepedia VP_71745
Entrez Gene 71745
UniProt Q9D4H8  
 Cul2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cul2
Molecular Function
    ubiquitin protein ligase binding GO:0031625 ISO
    protein complex binding GO:0032403 ISO
    protein binding GO:0005515 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    protein catabolic process GO:0030163 IMP
Subcellular Localization
    VCB complex GO:0030891 ISO
    cullin-RING ubiquitin ligase complex GO:0031461 IEA
    nucleoplasm GO:0005654 ISO
    Cul2-RING ubiquitin ligase complex GO:0031462 ISO
    nucleolus GO:0005730 IDA
 Experiment description of studies that identified Cul2 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cul2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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