Gene description for Cul2
Gene name cullin 2
Gene symbol Cul2
Other names/aliases 1300003D18Rik
4932411N15Rik
AI327301
mKIAA4106
Species Mus musculus
 Database cross references - Cul2
ExoCarta ExoCarta_71745
Vesiclepedia VP_71745
Entrez Gene 71745
UniProt Q9D4H8  
 Cul2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cul2
Molecular Function
    protein binding GO:0005515 IPI
    protein-macromolecule adaptor activity GO:0030674 IBA
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    ubiquitin ligase complex scaffold activity GO:0160072 IEA
    ubiquitin ligase complex scaffold activity GO:0160072 ISO
Biological Process
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IEA
    protein catabolic process GO:0030163 IMP
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IBA
    ubiquitin-dependent protein catabolic process via the C-end degron rule pathway GO:0140627 IEA
    ubiquitin-dependent protein catabolic process via the C-end degron rule pathway GO:0140627 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IDA
    SCF ubiquitin ligase complex GO:0019005 IBA
    VCB complex GO:0030891 IEA
    VCB complex GO:0030891 ISO
    Cul2-RING ubiquitin ligase complex GO:0031462 IBA
    Cul2-RING ubiquitin ligase complex GO:0031462 IDA
    Cul2-RING ubiquitin ligase complex GO:0031462 ISO
 Experiment description of studies that identified Cul2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cul2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rnf7  
Co-fractionation Mus musculus
2 Skp1a 21402
Co-fractionation Mus musculus
3 Ubc  
Reconstituted Complex Mus musculus
4 Myog  
Reconstituted Complex Mus musculus
5 Trmt112  
Co-fractionation Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Htt  
Affinity Capture-Western Mus musculus
8 VHL  
Affinity Capture-Western Homo sapiens
9 Unk  
Affinity Capture-RNA Mus musculus
10 Socs1  
Co-fractionation Mus musculus
View the network image/svg+xml



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