Gene description for Lonp1
Gene name lon peptidase 1, mitochondrial
Gene symbol Lonp1
Other names/aliases 1200017E13Rik
LON
Prss15
Species Mus musculus
 Database cross references - Lonp1
ExoCarta ExoCarta_74142
Vesiclepedia VP_74142
Entrez Gene 74142
UniProt Q8CGK3  
 Lonp1 identified in sEVs derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
Neural stem cells 25242146    
 Gene ontology annotations for Lonp1
Molecular Function
    mitochondrial promoter sequence-specific DNA binding GO:0001018 IDA
    mitochondrial promoter sequence-specific DNA binding GO:0001018 IDA
    single-stranded DNA binding GO:0003697 IBA
    single-stranded DNA binding GO:0003697 IDA
    single-stranded RNA binding GO:0003727 IEA
    single-stranded RNA binding GO:0003727 ISO
    ATP-dependent peptidase activity GO:0004176 IBA
    ATP-dependent peptidase activity GO:0004176 IDA
    ATP-dependent peptidase activity GO:0004176 ISO
    serine-type endopeptidase activity GO:0004252 IEA
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IDA
    PH domain binding GO:0042731 IPI
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    ADP binding GO:0043531 IEA
    ADP binding GO:0043531 ISO
    insulin receptor substrate binding GO:0043560 IPI
    sequence-specific DNA binding GO:0043565 ISO
    G-quadruplex DNA binding GO:0051880 IEA
    G-quadruplex DNA binding GO:0051880 ISO
    DNA polymerase binding GO:0070182 IEA
    DNA polymerase binding GO:0070182 ISO
Biological Process
    response to hypoxia GO:0001666 IEA
    proteolysis GO:0006508 IDA
    protein quality control for misfolded or incompletely synthesized proteins GO:0006515 IBA
    protein quality control for misfolded or incompletely synthesized proteins GO:0006515 IEA
    mitochondrion organization GO:0007005 IBA
    mitochondrion organization GO:0007005 IEA
    mitochondrion organization GO:0007005 ISO
    response to hormone GO:0009725 IEA
    response to aluminum ion GO:0010044 IEA
    protein catabolic process GO:0030163 ISO
    protein catabolic process GO:0030163 ISS
    cellular response to oxidative stress GO:0034599 IEA
    cellular response to oxidative stress GO:0034599 ISO
    negative regulation of insulin receptor signaling pathway GO:0046627 IDA
    regulation of peptidyl-tyrosine phosphorylation GO:0050730 IDA
    chaperone-mediated protein complex assembly GO:0051131 IBA
    chaperone-mediated protein complex assembly GO:0051131 IEA
    proteolysis involved in protein catabolic process GO:0051603 ISO
    protein-containing complex assembly GO:0065003 ISO
    oxidation-dependent protein catabolic process GO:0070407 IEA
    oxidation-dependent protein catabolic process GO:0070407 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IBA
    mitochondrial matrix GO:0005759 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    mitochondrial nucleoid GO:0042645 IEA
    mitochondrial nucleoid GO:0042645 ISO
 Experiment description of studies that identified Lonp1 in sEVs
1
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Lonp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Slc27a2  
Co-fractionation Mus musculus
2 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Lars2  
Co-fractionation Mus musculus
5 Lonp1 74142
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
6 Xpo5  
Co-fractionation Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Lonp1 is involved
PathwayEvidenceSource
Metabolism of proteins IEA Reactome
Mitochondrial protein degradation IEA Reactome





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