Gene description for ALPK1
Gene name alpha-kinase 1
Gene symbol ALPK1
Other names/aliases 8430410J10Rik
LAK
Species Homo sapiens
 Database cross references - ALPK1
ExoCarta ExoCarta_80216
Vesiclepedia VP_80216
Entrez Gene 80216
HGNC 20917
MIM 607347
UniProt Q96QP1  
 ALPK1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ALPK1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 EXP
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    monosaccharide binding GO:0048029 IBA
    monosaccharide binding GO:0048029 IPI
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    cytoplasmic pattern recognition receptor signaling pathway GO:0002753 IBA
    cytoplasmic pattern recognition receptor signaling pathway GO:0002753 IDA
    cytoplasmic pattern recognition receptor signaling pathway GO:0002753 TAS
    chromatin remodeling GO:0006338 IEA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IDA
    innate immune response GO:0045087 IBA
    innate immune response GO:0045087 IDA
    cilium assembly GO:0060271 IMP
Subcellular Localization
    spindle pole GO:0000922 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cilium GO:0005929 IBA
    cilium GO:0005929 IDA
 Experiment description of studies that identified ALPK1 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ALPK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD1B 910
Affinity Capture-MS Homo sapiens
2 SCGB1D2  
Affinity Capture-MS Homo sapiens
3 MYC  
Dosage Lethality Homo sapiens
4 ALOXE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
6 XPO1 7514
Affinity Capture-MS Homo sapiens
7 PIP 5304
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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