Gene description for PIGY
Gene name phosphatidylinositol glycan anchor biosynthesis, class Y
Gene symbol PIGY
Other names/aliases PIG-Y
Species Homo sapiens
 Database cross references - PIGY
ExoCarta ExoCarta_84992
Vesiclepedia VP_84992
Entrez Gene 84992
HGNC 28213
MIM 610662
UniProt Q3MUY2  
 PIGY identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for PIGY
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol N-acetylglucosaminyltransferase activity GO:0017176 IDA
Biological Process
    GPI anchor biosynthetic process GO:0006506 IBA
    GPI anchor biosynthetic process GO:0006506 IDA
    GPI anchor biosynthetic process GO:0006506 IEA
Subcellular Localization
    glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex GO:0000506 IBA
    glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex GO:0000506 IDA
    glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex GO:0000506 IPI
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified PIGY in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for PIGY
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ABCB4 5244
Two-hybrid Homo sapiens
2 C14orf1 11161
Two-hybrid Homo sapiens
3 WBSCR16  
Affinity Capture-MS Homo sapiens
4 TMEM72  
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here