Gene description for Picalm
Gene name phosphatidylinositol binding clathrin assembly protein
Gene symbol Picalm
Other names/aliases Calm
Species Rattus norvegicus
 Database cross references - Picalm
ExoCarta ExoCarta_89816
Entrez Gene 89816
UniProt O55012  
 Picalm identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Picalm
Molecular Function
    clathrin heavy chain binding GO:0032050 ISO
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    1-phosphatidylinositol binding GO:0005545 IEA
    clathrin binding GO:0030276 ISS
    clathrin adaptor activity GO:0035615 ISS
    SH3 domain binding GO:0017124 IDA
Biological Process
    regulation of endocytosis GO:0030100 ISO
    vesicle-mediated transport GO:0016192 IMP
    positive regulation of neuron death GO:1901216 ISS
    negative regulation of gene expression GO:0010629 ISS
    positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process GO:1902961 ISS
    cargo loading into vesicle GO:0035459 ISS
    positive regulation of beta-amyloid formation GO:1902004 ISS
    iron ion import into cell GO:0097459 ISS
    synaptic vesicle maturation GO:0016188 ISS
    cell proliferation GO:0008283 ISS
    endosomal transport GO:0016197 ISO
    receptor-mediated endocytosis GO:0006898 ISS
    regulation of protein transport GO:0051223 IMP
    clathrin coat assembly GO:0048268 ISO
    hemopoiesis GO:0030097 ISO
    regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process GO:1902959 ISS
    axonogenesis GO:0007409 ISO
    negative regulation of receptor-mediated endocytosis GO:0048261 ISO
    dendrite morphogenesis GO:0048813 ISO
    receptor internalization GO:0031623 ISO
    iron ion homeostasis GO:0055072 ISS
    negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process GO:1902963 ISS
    clathrin-mediated endocytosis GO:0072583 ISS
    regulation of protein localization GO:0032880 ISO
    positive regulation of transcription, DNA-templated GO:0045893 ISO
Subcellular Localization
    intracellular membrane-bounded organelle GO:0043231 ISO
    coated pit GO:0005905 ISS
    plasma membrane GO:0005886 IDA
    neuronal postsynaptic density GO:0097481 IDA
    synaptic vesicle GO:0008021 IDA
    nucleus GO:0005634 ISO
    synapse GO:0045202 IDA
    intracellular GO:0005622 ISO
    endosome GO:0005768 IDA
    Golgi apparatus GO:0005794 IEA
    clathrin-coated vesicle GO:0030136 IDA
    vesicle GO:0031982 ISS
    perinuclear region of cytoplasm GO:0048471 ISO
    neuronal cell body GO:0043025 ISS
    clathrin coat of coated pit GO:0030132 ISO
    neurofibrillary tangle GO:0097418 ISS
    membrane GO:0016020 ISO
    presynaptic membrane GO:0042734 IDA
    postsynaptic membrane GO:0045211 IDA
    AP-2 adaptor complex GO:0030122 ISO
 Experiment description of studies that identified Picalm in exosomes
1
Experiment ID 95
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Picalm
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ehd2 361512
Reconstituted Complex Rattus norvegicus
View the network image/svg+xml
 Pathways in which Picalm is involved
No pathways found





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