Gene description for Prdx6
Gene name peroxiredoxin 6
Gene symbol Prdx6
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Prdx6
ExoCarta ExoCarta_94167
Entrez Gene 94167
UniProt O35244  
 Prdx6 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Prdx6
Molecular Function
    peroxiredoxin activity GO:0051920 IEA
    glutathione peroxidase activity GO:0004602 IDA
    protein binding GO:0005515 IPI
    peroxidase activity GO:0004601 ISO
    phospholipase A2 activity GO:0004623 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
Biological Process
    phospholipid catabolic process GO:0009395 ISO
    response to reactive oxygen species GO:0000302 ISO
    bleb assembly GO:0032060 ISO
    oxidation-reduction process GO:0055114 ISO
    response to oxidative stress GO:0006979 ISO
    hydrogen peroxide catabolic process GO:0042744 IDA
Subcellular Localization
    membrane GO:0016020 ISO
    cytoplasm GO:0005737 ISS
    extracellular exosome GO:0070062 ISO
    cytosol GO:0005829 ISO
    cytoplasmic membrane-bounded vesicle GO:0016023 IEA
    extracellular space GO:0005615 ISO
    lysosome GO:0005764 IEA
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Prdx6 in exosomes
1
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 192
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
CD63|CD81|FLOT
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Prdx6
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prdx6 is involved
PathwayEvidenceSource
Detoxification of Reactive Oxygen Species IEA Reactome





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