Gene description for CHST2
Gene name carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2
Gene symbol CHST2
Other names/aliases C6ST
GST-2
GST2
Gn6ST-1
HEL-S-75
glcNAc6ST-1
Species Homo sapiens
 Database cross references - CHST2
ExoCarta ExoCarta_9435
Vesiclepedia VP_9435
Entrez Gene 9435
HGNC 1970
MIM 603798
UniProt Q9Y4C5  
 CHST2 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for CHST2
Molecular Function
    N-acetylglucosamine 6-O-sulfotransferase activity GO:0001517 IBA
    N-acetylglucosamine 6-O-sulfotransferase activity GO:0001517 IDA
    N-acetylglucosamine 6-O-sulfotransferase activity GO:0001517 TAS
    sulfotransferase activity GO:0008146 TAS
Biological Process
    carbohydrate metabolic process GO:0005975 IEA
    N-acetylglucosamine metabolic process GO:0006044 IBA
    N-acetylglucosamine metabolic process GO:0006044 IDA
    sulfur compound metabolic process GO:0006790 IBA
    sulfur compound metabolic process GO:0006790 IDA
    inflammatory response GO:0006954 IEA
    keratan sulfate biosynthetic process GO:0018146 TAS
    positive regulation of leukocyte tethering or rolling GO:1903238 ISS
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    Golgi membrane GO:0000139 TAS
    nucleoplasm GO:0005654 IDA
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 IBA
    trans-Golgi network GO:0005802 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified CHST2 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for CHST2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPA5 3309
Affinity Capture-MS Homo sapiens
2 RNF208  
Affinity Capture-MS Homo sapiens
3 CANX 821
Affinity Capture-MS Homo sapiens
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