Gene description for CDK6
Gene name cyclin-dependent kinase 6
Gene symbol CDK6
Other names/aliases MCPH12
PLSTIRE
Species Homo sapiens
 Database cross references - CDK6
ExoCarta ExoCarta_1021
Vesiclepedia VP_1021
Entrez Gene 1021
HGNC 1777
MIM 603368
UniProt Q00534  
 CDK6 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Neuroblastoma cells 25944692    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Prostate cancer cells 25844599    
 Gene ontology annotations for CDK6
Molecular Function
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IBA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    cyclin binding GO:0030332 IBA
    cyclin binding GO:0030332 IPI
    FBXO family protein binding GO:0098770 IPI
    protein serine kinase activity GO:0106310 IEA
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 IBA
    G1/S transition of mitotic cell cycle GO:0000082 IDA
    G1/S transition of mitotic cell cycle GO:0000082 NAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    positive regulation of cell-matrix adhesion GO:0001954 IDA
    type B pancreatic cell development GO:0003323 IDA
    protein phosphorylation GO:0006468 IDA
    signal transduction GO:0007165 IBA
    Notch signaling pathway GO:0007219 IEA
    negative regulation of cell population proliferation GO:0008285 TAS
    response to virus GO:0009615 IEP
    regulation of G2/M transition of mitotic cell cycle GO:0010389 IBA
    regulation of gene expression GO:0010468 IBA
    regulation of gene expression GO:0010468 IDA
    regulation of gene expression GO:0010468 IMP
    positive regulation of gene expression GO:0010628 IEA
    astrocyte development GO:0014002 ISS
    dentate gyrus development GO:0021542 ISS
    lateral ventricle development GO:0021670 ISS
    T cell differentiation in thymus GO:0033077 IEA
    gliogenesis GO:0042063 IMP
    cell dedifferentiation GO:0043697 IMP
    negative regulation of cell differentiation GO:0045596 TAS
    negative regulation of myeloid cell differentiation GO:0045638 IDA
    regulation of erythrocyte differentiation GO:0045646 IMP
    negative regulation of monocyte differentiation GO:0045656 IDA
    negative regulation of osteoblast differentiation GO:0045668 IDA
    negative regulation of cell cycle GO:0045786 IDA
    positive regulation of fibroblast proliferation GO:0048146 IMP
    generation of neurons GO:0048699 ISS
    negative regulation of epithelial cell proliferation GO:0050680 IMP
    cell division GO:0051301 IEA
    regulation of cell cycle GO:0051726 TAS
    hematopoietic stem cell differentiation GO:0060218 IEA
    regulation of hematopoietic stem cell differentiation GO:1902036 IEA
    regulation of cell motility GO:2000145 ISS
    negative regulation of cellular senescence GO:2000773 IDA
Subcellular Localization
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IBA
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IDA
    ruffle GO:0001726 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cyclin D1-CDK6 complex GO:0097131 IPI
    cyclin D2-CDK6 complex GO:0097132 IEA
    cyclin D3-CDK6 complex GO:0097133 IPI
 Experiment description of studies that identified CDK6 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
12
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
13
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
17
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for CDK6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 PSMA3 5684
Two-hybrid Homo sapiens
3 SENP3 26168
Biochemical Activity Homo sapiens
4 ZNF174  
Biochemical Activity Homo sapiens
5 AMY2A 279
Affinity Capture-MS Homo sapiens
6 CCNH  
Two-hybrid Homo sapiens
7 FBXO7 25793
Affinity Capture-Western Homo sapiens
8 KLC3  
Affinity Capture-MS Homo sapiens
9 BCL11A 53335
Biochemical Activity Homo sapiens
10 EPB41L5 57669
Affinity Capture-MS Homo sapiens
11 DAB1 1600
Two-hybrid Homo sapiens
12 NUMA1 4926
Biochemical Activity Homo sapiens
13 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
14 VHL  
Negative Genetic Homo sapiens
15 CBY1  
Biochemical Activity Homo sapiens
16 SSBP2  
Biochemical Activity Homo sapiens
17 ZFP36  
Biochemical Activity Homo sapiens
18 SOX10  
Biochemical Activity Homo sapiens
19 FGFR3 2261
Negative Genetic Homo sapiens
20 FAM26E  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CCNI 10983
Two-hybrid Homo sapiens
22 EIF4ENIF1 56478
Biochemical Activity Homo sapiens
23 OTP  
Two-hybrid Homo sapiens
24 PAK4 10298
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 USP17L2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
26 MCM2 4171
Reconstituted Complex Homo sapiens
27 MEF2D  
Biochemical Activity Homo sapiens
28 NFATC3 4775
Biochemical Activity Homo sapiens
29 CDKN1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TCEB3B  
Biochemical Activity Homo sapiens
31 UBE2W  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 APOBEC1  
Two-hybrid Homo sapiens
33 SOX5 6660
Biochemical Activity Homo sapiens
34 TRIM15  
Two-hybrid Homo sapiens
35 MSL3  
Biochemical Activity Homo sapiens
36 SORBS1 10580
Biochemical Activity Homo sapiens
37 ANKRD12  
Biochemical Activity Homo sapiens
38 MCF2L2 23101
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 RUNX1  
Co-localization Homo sapiens
40 RB1 5925
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
41 PPM1B 5495
Biochemical Activity Homo sapiens
Co-purification Homo sapiens
42 FAM96A  
Affinity Capture-MS Homo sapiens
43 HSP90AA5P 730211
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 BSPRY 54836
Affinity Capture-MS Homo sapiens
45 RTFDC1  
Affinity Capture-MS Homo sapiens
46 CDKN1A  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 TRA2A 29896
Biochemical Activity Homo sapiens
48 VGLL4  
Biochemical Activity Homo sapiens
49 MCM10 55388
Reconstituted Complex Homo sapiens
50 ZMYM3  
Biochemical Activity Homo sapiens
51 ACACA 31
Positive Genetic Homo sapiens
52 TFDP1  
Biochemical Activity Homo sapiens
53 CDH1 999
Affinity Capture-Western Homo sapiens
54 TPX2  
Biochemical Activity Homo sapiens
55 RPA2 6118
Proximity Label-MS Homo sapiens
56 NTRK1 4914
Affinity Capture-MS Homo sapiens
57 CTNNB1 1499
Co-localization Homo sapiens
58 PPP2CA 5515
Co-purification Homo sapiens
Biochemical Activity Homo sapiens
59 CDK5R1  
Affinity Capture-Western Homo sapiens
60 KLF10  
Biochemical Activity Homo sapiens
61 PML 5371
Biochemical Activity Homo sapiens
62 ISL1  
Biochemical Activity Homo sapiens
63 EIF2AK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 CCNT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 RBL1  
Biochemical Activity Homo sapiens
66 ATXN1 6310
Two-hybrid Homo sapiens
67 PHLPP1  
Proximity Label-MS Homo sapiens
68 SRSF11 9295
Biochemical Activity Homo sapiens
69 PRX 57716
Biochemical Activity Homo sapiens
70 ZNF101  
Biochemical Activity Homo sapiens
71 EBF4  
Biochemical Activity Homo sapiens
72 CCNA2 890
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
73 PPM1M  
Affinity Capture-MS Homo sapiens
74 CDK7 1022
Biochemical Activity Homo sapiens
75 ZSCAN1  
Biochemical Activity Homo sapiens
76 SRSF1 6426
Biochemical Activity Homo sapiens
77 WDR33 55339
Affinity Capture-MS Homo sapiens
78 SYNPO2  
Biochemical Activity Homo sapiens
79 CCNE1  
Affinity Capture-Western Homo sapiens
80 UHRF2  
Far Western Homo sapiens
81 FARP2 9855
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
82 PTK2 5747
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
83 METTL21B  
Affinity Capture-MS Homo sapiens
84 MYC  
Biochemical Activity Homo sapiens
85 LPIN1 23175
Biochemical Activity Homo sapiens
86 KIF26B  
Biochemical Activity Homo sapiens
87 PPHLN1  
Biochemical Activity Homo sapiens
88 CCND3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
89 ELK1  
Biochemical Activity Homo sapiens
90 CDK2 1017
Co-purification Homo sapiens
Co-localization Homo sapiens
Co-fractionation Homo sapiens
91 FKBP5 2289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 TJP2 9414
Biochemical Activity Homo sapiens
93 DEDD2  
Biochemical Activity Homo sapiens
94 RBL2 5934
Biochemical Activity Homo sapiens
95 CLASRP  
Biochemical Activity Homo sapiens
96 NIPBL 25836
Biochemical Activity Homo sapiens
97 HIST1H1A 3024
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
98 CDKN2D  
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
99 CDKN1B 1027
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 CCND1 595
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
101 MZF1  
Biochemical Activity Homo sapiens
102 CDKN2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
103 PIGR 5284
Affinity Capture-MS Homo sapiens
104 SUMO1 7341
Biochemical Activity Homo sapiens
105 DMBT1 1755
Affinity Capture-MS Homo sapiens
106 FASN 2194
Positive Genetic Homo sapiens
107 FOXO3  
Biochemical Activity Homo sapiens
108 CCND2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
109 TRAK1 22906
Biochemical Activity Homo sapiens
110 CDKL3  
Affinity Capture-MS Homo sapiens
111 CA4 762
Affinity Capture-MS Homo sapiens
112 ZEB1  
Biochemical Activity Homo sapiens
113 CDC37 11140
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
114 SRSF2 6427
Biochemical Activity Homo sapiens
115 CDC6  
Biochemical Activity Homo sapiens
116 ZNF335 63925
Biochemical Activity Homo sapiens
117 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
118 RCHY1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
120 CDK6 1021
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 TRA2B 6434
Biochemical Activity Homo sapiens
122 SIRT1  
Biochemical Activity Homo sapiens
123 CDK1 983
Co-fractionation Homo sapiens
124 PCNA 5111
Affinity Capture-MS Homo sapiens
125 ATF6B  
Biochemical Activity Homo sapiens
126 RBM23  
Biochemical Activity Homo sapiens
127 DDIT3 1649
Biochemical Activity Homo sapiens
128 ABI2 10152
Biochemical Activity Homo sapiens
129 SNIP1  
Biochemical Activity Homo sapiens
130 EZH2  
Biochemical Activity Homo sapiens
131 EGLN2  
Affinity Capture-Western Homo sapiens
132 CDK4 1019
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
133 HSF1  
Biochemical Activity Homo sapiens
134 SRSF7 6432
Biochemical Activity Homo sapiens
135 FOXM1  
Biochemical Activity Homo sapiens
136 MLLT3 4300
Biochemical Activity Homo sapiens
137 SERBP1 26135
Affinity Capture-MS Homo sapiens
138 NEU2  
Affinity Capture-MS Homo sapiens
139 SLBP  
Biochemical Activity Homo sapiens
140 AR 367
Co-localization Homo sapiens
141 CDKN2B 1030
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
142 CCNT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 POGZ 23126
Biochemical Activity Homo sapiens
144 PPARGC1A  
Biochemical Activity Homo sapiens
145 SRSF12  
Biochemical Activity Homo sapiens
146 UBE2I 7329
Affinity Capture-Western Homo sapiens
147 CDKN2A 1029
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
148 CASC3  
Biochemical Activity Homo sapiens
149 N4BP1  
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which CDK6 is involved
PathwayEvidenceSource
Aberrant regulation of mitotic cell cycle due to RB1 defects TAS Reactome
Aberrant regulation of mitotic G1/S transition in cancer due to RB1 defects TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Cyclin D associated events in G1 TAS Reactome
Defective binding of RB1 mutants to E2F1,(E2F2, E2F3) TAS Reactome
Disease TAS Reactome
Diseases of cellular response to stress TAS Reactome
Diseases of Cellular Senescence TAS Reactome
Diseases of mitotic cell cycle TAS Reactome
Drug-mediated inhibition of CDK4/CDK6 activity TAS Reactome
Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 TAS Reactome
Evasion of Oncogene Induced Senescence Due to p16INK4A Defects TAS Reactome
Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 TAS Reactome
Evasion of Oxidative Stress Induced Senescence Due to p16INK4A Defects TAS Reactome
G1 Phase TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Mitotic G1 phase and G1/S transition TAS Reactome
Oncogene Induced Senescence TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome
Regulation of RUNX1 Expression and Activity TAS Reactome
RNA Polymerase II Transcription TAS Reactome
Senescence-Associated Secretory Phenotype (SASP) TAS Reactome
Transcriptional regulation by RUNX1 TAS Reactome





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