Gene ontology annotations for DNM1
Experiment description of studies that identified DNM1 in sEVs
1
Experiment ID
489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
363
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
6
Experiment ID
364
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
7
Experiment ID
365
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
8
Experiment ID
224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
9
Experiment ID
231
MISEV standards
✘
Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
10
Experiment ID
232
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
11
Experiment ID
233
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
12
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
13
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
Protein-protein interactions for DNM1
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
GPM6A
2823
Affinity Capture-MS
Homo sapiens
2
PACSIN1
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
3
TBC1D2
55357
Co-fractionation
Homo sapiens
4
SNX33
257364
Affinity Capture-MS
Homo sapiens
5
SH3BP4
23677
Co-localization
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
6
SH3GL1
6455
Reconstituted Complex
Homo sapiens
7
PACSIN2
11252
Reconstituted Complex
Homo sapiens
8
TYROBP
Affinity Capture-MS
Homo sapiens
9
Dyrk1a
Biochemical Activity
Rattus norvegicus
10
LOC100132735
Protein-RNA
Homo sapiens
11
AMIGO1
Affinity Capture-MS
Homo sapiens
12
DYRK1A
1859
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
13
NUFIP1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
14
SRC
6714
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
15
FER
2241
Co-fractionation
Homo sapiens
16
TBC1D15
64786
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
17
PFN2
5217
Co-fractionation
Homo sapiens
18
MECP2
4204
Affinity Capture-MS
Homo sapiens
19
DNM1
1759
Co-crystal Structure
Homo sapiens
Two-hybrid
Homo sapiens
Co-crystal Structure
Homo sapiens
Two-hybrid
Homo sapiens
20
NCK1
4690
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
21
HSPA8
3312
Co-fractionation
Homo sapiens
22
BSG
682
Affinity Capture-MS
Homo sapiens
23
CD70
970
Affinity Capture-MS
Homo sapiens
24
KLRC4
Affinity Capture-MS
Homo sapiens
25
Asap1
Two-hybrid
Mus musculus
26
CABIN1
Affinity Capture-Western
Homo sapiens
27
CDK5
1020
Biochemical Activity
Homo sapiens
28
IRF2BPL
64207
Co-fractionation
Homo sapiens
29
SLC39A9
55334
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
30
RPE
Co-fractionation
Homo sapiens
31
UBE2I
7329
Two-hybrid
Homo sapiens
32
CDKAL1
Affinity Capture-MS
Homo sapiens
33
NTRK1
4914
Affinity Capture-MS
Homo sapiens
34
PTK2B
2185
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
35
LYPD6
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
36
FNBP1
23048
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
37
DNM2
1785
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
38
MED31
Two-hybrid
Homo sapiens
39
BIN1
274
Protein-peptide
Homo sapiens
40
DNAAF2
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
41
ZBBX
Affinity Capture-MS
Homo sapiens
42
EFNA4
Affinity Capture-MS
Homo sapiens
43
CLINT1
9685
Reconstituted Complex
Homo sapiens
44
FANCD2
Co-fractionation
Homo sapiens
45
IQCB1
Affinity Capture-MS
Homo sapiens
46
TMEM9B
56674
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
47
NPM3
10360
Co-fractionation
Homo sapiens
48
Amph
Affinity Capture-Western
Rattus norvegicus
49
PACSIN3
29763
Reconstituted Complex
Homo sapiens
50
KIAA1377
Two-hybrid
Homo sapiens
51
AQP3
Affinity Capture-MS
Homo sapiens
52
SNX9
51429
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
53
AMPH
273
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
54
AURKB
9212
Affinity Capture-MS
Homo sapiens
55
DMAP1
55929
Affinity Capture-MS
Homo sapiens
56
ANKRD22
Affinity Capture-MS
Homo sapiens
57
PIAS1
8554
Two-hybrid
Homo sapiens
58
UBASH3A
53347
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
59
Eps15
13858
Reconstituted Complex
Mus musculus
60
SCN2B
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
61
HAX1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
62
MRPL38
Affinity Capture-MS
Homo sapiens
63
MAP3K10
Reconstituted Complex
Homo sapiens
64
SH3GL2
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
65
PIN4
5303
Two-hybrid
Homo sapiens
66
SUMO1
7341
Two-hybrid
Homo sapiens
Co-crystal Structure
Homo sapiens
67
FTL
2512
Affinity Capture-MS
Homo sapiens
68
GYPA
Affinity Capture-MS
Homo sapiens
69
PLEKHA4
57664
Affinity Capture-MS
Homo sapiens
70
TMEM9
252839
Affinity Capture-MS
Homo sapiens
71
PARP1
142
Affinity Capture-MS
Homo sapiens
72
ITSN1
6453
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
73
GRB2
2885
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Far Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
74
MFSD4
Affinity Capture-MS
Homo sapiens
75
Fcho2
Affinity Capture-Western
Mus musculus
76
Ap2a1
Affinity Capture-Western
Rattus norvegicus
77
Grb2
Affinity Capture-Western
Rattus norvegicus
78
ASB13
Two-hybrid
Homo sapiens
79
DNMBP
23268
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
80
NCK2
8440
Affinity Capture-Western
Homo sapiens
81
NCL
4691
Co-fractionation
Homo sapiens
82
ARRB1
408
Affinity Capture-MS
Homo sapiens
83
DNM3
26052
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
84
SSSCA1
10534
Affinity Capture-MS
Homo sapiens
85
DGUOK
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
86
ASCL4
Affinity Capture-MS
Homo sapiens
87
SH3KBP1
30011
Affinity Capture-MS
Homo sapiens
88
SNRPA1
6627
Co-fractionation
Homo sapiens
89
RPA3
6119
Proximity Label-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which DNM1 is involved